Parent D, Golstein J, Mairesse N, Bernerd F, Faverly D, Robben H, Ramaekers F, Heenen M
Institute for Interdisciplinary Research in Human and Nuclear Biology, School of Medicine, Free University of Brussels, Belgium.
Cell Prolif. 1994 Mar;27(3):165-76. doi: 10.1111/j.1365-2184.1994.tb01414.x.
A murine monoclonal antibody, FB1, reacted with the basal keratinocytes of human stratified epithelia. One-dimensional and two-dimensional immunoblotting assays, performed on keratins extracted from HaCat cells and normal human keratinocytes, showed that FB1 recognizes K14. When LL002, another K14 monoclonal antibody is added, the FB1 stained area in the 2D-immunoblot seems to cover a fraction of the LL002 spot. Immunohistochemical data obtained from studies on normal human tissues supported the K14 specificity of FB1, but when compared with two other monoclonal antibodies, LL002 and RCK107 reacting with K14, some differences appeared. These differences were mainly seen in sweat glands, hair follicles, psoriatic epidermis and salivary glands. In psoriatic epidermis, FB1 showed a heterogeneous pattern of staining of the basal cell compartment. Intense reactivity was only observed at the bottom of the rete ridges. Staining diminished and finally disappeared in the basal cells above the dermal papillae. This observation supports the view that an increased germinative cell population in psoriasis involves a partially differentiated amplifying compartment in which the number of cell divisions is increased.
一种鼠单克隆抗体FB1与人复层上皮的基底角质形成细胞发生反应。对从HaCaT细胞和正常人角质形成细胞中提取的角蛋白进行的一维和二维免疫印迹分析表明，FB1识别K14。当加入另一种K14单克隆抗体LL002时，二维免疫印迹中FB1染色区域似乎覆盖了LL002斑点的一部分。对正常人体组织研究获得的免疫组织化学数据支持FB1对K14的特异性，但与另外两种与K14反应的单克隆抗体LL002和RCK107相比，出现了一些差异。这些差异主要见于汗腺、毛囊、银屑病表皮和唾液腺。在银屑病表皮中，FB1在基底细胞区显示出异质性染色模式。仅在 rete 嵴底部观察到强烈反应性。在真皮乳头上方的基底细胞中染色减弱并最终消失。这一观察结果支持了这样一种观点，即银屑病中增殖细胞群的增加涉及一个部分分化的扩增区室，其中细胞分裂数量增加。
