Manderfeld M M, Schafer H W, Davidson P M, Zottola E A
Department of Food Science and Nutrition, University of Minnesota, St. Paul 55108, USA.
J Food Prot. 1997 Jan;60(1):72-7. doi: 10.4315/0362-028x-60.1.72.
Photoactive furocoumarins extracted from four varieties of fresh and freeze-dried parsley leaves inhibited a DNA repair-deficient Escherichia coli in a photobiological assay. Using media-modified assays, the human pathogens E. coli O157:H7 and Listeria monocytogenes, the spoilage microorganism Erwinia carotovora, and Listeria innocua were also inhibited. Pseudomonas fragi was not inhibited. Minimum concentrations of Forest Green parsley powder in agar which showed inhibition ranged from 0.12% to 8.0% depending on the microorganism. Ultraviolet light (UV) at 365 nm for 60 min used to photoactivate the furocoumarins in the bioassay had little effect on L. monocytogenes and L. innocua. A slight UV inhibitory effect was detected with E. carotovora. Furocoumarins, psoralen, 8-methoxypsoralen (8-MOP), 5-methoxypsoralen (5-MOP), oxypeucedanin and isopimpinellin were identified using gas chromatography-mass spectrometry. Psoralen, 8-MOP, and 5-MOP were quantified. A difference in relative furocoumarin concentration (weight of furocoumarin per weight of dry parsley leaves) for all varieties of parsley was revealed. The concentration of 5-MOP was significantly greater than 8-MOP (P < 0.05), but not significantly greater than psoralen. Psoralen and 8-MOP were not significantly different in concentration.
从四种新鲜和冻干的欧芹叶中提取的光活性呋喃香豆素在光生物学试验中抑制了一种DNA修复缺陷型大肠杆菌。使用改良培养基试验，还抑制了人类病原体大肠杆菌O157:H7和单核细胞增生李斯特菌、腐败微生物胡萝卜软腐欧文氏菌以及无害李斯特菌。脆假单胞菌未被抑制。琼脂中显示出抑制作用的森林绿欧芹粉的最低浓度因微生物而异，范围为0.12%至8.0%。在生物测定中用于光激活呋喃香豆素的365 nm紫外线照射60分钟对单核细胞增生李斯特菌和无害李斯特菌几乎没有影响。在胡萝卜软腐欧文氏菌中检测到轻微的紫外线抑制作用。使用气相色谱-质谱法鉴定了呋喃香豆素、补骨脂素、8-甲氧基补骨脂素（8-MOP）、5-甲氧基补骨脂素（5-MOP）、氧化前胡素和异茴芹内酯。对补骨脂素、8-MOP和5-MOP进行了定量。揭示了所有品种欧芹相对呋喃香豆素浓度（每干欧芹叶重量中呋喃香豆素的重量）的差异。5-MOP的浓度显著高于8-MOP（P < 0.05），但不显著高于补骨脂素。补骨脂素和8-MOP的浓度没有显著差异。
