Salman Haya Ayyal, Yaakop Amira Suriaty, Al-Mustafa Ahmed, Tarawneh Khaled, Aladaileh Saleem, Al-Rimawi Fuad, Alakhras Fadi, Abu-Lafi Saleh, Zarzour Raghdaa Al, Wahab Habibah
School of Biological Sciences, Universiti Sains Malaysia, 11800 Penang, Malaysia.
Department of Biological Sciences, Faculty of Science, Mutah University, P.O. Box 7, Mutah 61710, Al-Karak, Jordan.
Saudi J Biol Sci. 2021 Nov;28(11):6245-6253. doi: 10.1016/j.sjbs.2021.06.090. Epub 2021 Jul 6.
Screening of phytochemical crude extract by GC-MS and HPLC analysis indicated the presence of alkaloids, tannins, flavonoids, terpenoids, and phenolic acid in the extract. The total phenolic content of methanol extract was 39.43 mg of Gallic acid eq/g, crude with 56.74, and 2.42 µg Trolox equivalent antioxidant capacity (TEAC)/g of plant extract according to DPPH and FRAP assay, respectively. The antimicrobial activity of against and demonstrated a mean zone diameter of inhibition ranging from 0 to 17 mm. The MIC of the extracts ranged from 0.5 to 1.0 mg/mL. extract inhibits pepsin enzyme activity with IC50 values of 213.67 µg/ml. This study revealed that extract has pepsin enzyme inhibitory, antibacterial, antioxidant activities. The current outcomes indicate that might be employed as a natural agent for managing GERD and infectious diseases.
通过气相色谱-质谱联用（GC-MS）和高效液相色谱（HPLC）分析对植物化学粗提物进行筛选，结果表明提取物中存在生物碱、单宁、黄酮类化合物、萜类化合物和酚酸。根据二苯基苦味酰基自由基（DPPH）和铁离子还原抗氧化能力（FRAP）测定，甲醇提取物的总酚含量分别为39.43毫克没食子酸当量/克、粗提取物为56.74毫克没食子酸当量/克，植物提取物的抗氧化能力为2.42微克特洛克斯当量（TEAC）/克。提取物对[具体菌种1]和[具体菌种2]的抗菌活性显示平均抑菌圈直径为0至17毫米。提取物的最低抑菌浓度（MIC）范围为0.5至1.0毫克/毫升。[植物名称]提取物以213.67微克/毫升的半数抑制浓度（IC50）值抑制胃蛋白酶活性。本研究表明，[植物名称]提取物具有胃蛋白酶抑制、抗菌和抗氧化活性。目前的结果表明，[植物名称]可能用作治疗胃食管反流病（GERD）和传染病的天然药物。
