Fogel M, Friederichs J, Zeller Y, Husar M, Smirnov A, Roitman L, Altevogt P, Sthoeger Z M
Tumor Immunology Research Laboratory, Kaplan Hospital, Rehovot, Israel.
Cancer Lett. 1999 Aug 23;143(1):87-94. doi: 10.1016/s0304-3835(99)00195-0.
CD24 is a small, mucin-type glycosylphosphatidylinositol-linked cell surface molecule expressed by neutrophils, pre B lymphocytes and certain human tumor cell lines. CD24 has been identified as a ligand for P-selectin in both mouse and human cells. We previously reported that the P-selectin-CD24 binding pathway is important for the binding of the breast carcinoma cell line KS to platelets and the rolling of these cells on endothelial P-selectin. In the present study we have analyzed the expression of CD24 on human breast carcinoma cell lines and on fresh breast carcinoma specimens using the CD24-specific antibody ML-5. Our study clearly demonstrates that CD24 is abundantly expressed on cell lines and fresh tissues of breast carcinomas. We find a differential expression of CD24 in breast carcinomas (cytoplasmic pattern) versus benign breast lesions (apical pattern). Moreover, the intensity of CD24 expression increases with the histological grade of the tumor. Thus, CD24 expression might be a useful marker for human breast carcinoma and play a role in facilitating metastasis by the interaction between tumor cells and platelets or endothelial cells.
CD24是一种小分子、粘蛋白型糖基磷脂酰肌醇连接的细胞表面分子，由中性粒细胞、前B淋巴细胞和某些人类肿瘤细胞系表达。在小鼠和人类细胞中，CD24已被确定为P-选择素的配体。我们之前报道过，P-选择素-CD24结合途径对于乳腺癌细胞系KS与血小板的结合以及这些细胞在内皮P-选择素上的滚动很重要。在本研究中，我们使用CD24特异性抗体ML-5分析了CD24在人乳腺癌细胞系和新鲜乳腺癌标本上的表达。我们的研究清楚地表明，CD24在乳腺癌的细胞系和新鲜组织中大量表达。我们发现CD24在乳腺癌（细胞质模式）与良性乳腺病变（顶端模式）中存在差异表达。此外，CD24表达强度随肿瘤组织学分级增加而增加。因此，CD24表达可能是人类乳腺癌的一个有用标志物，并通过肿瘤细胞与血小板或内皮细胞之间的相互作用在促进转移中发挥作用。
