Reglödi D, Lubics A, Szelier M, Lengvári I
Department of Anatomy, University Medical School, Pécs, Hungary.
Peptides. 1999;20(5):569-77. doi: 10.1016/s0196-9781(99)00009-1.
The distribution of cholecystokinin and gastrin-like immunoreactive cell bodies and fibers in the nervous system of 2 annelid worms, Lumbricus terrestris and Eisenia fetida, has been studied by means of immunohistochemistry. The cerebral ganglion contains 170-250, the subesophageal ganglion contains 120-150, and the ventral ganglia contain 50-75 cholecystokinin immunoreactive cells, that represent 8-12%, 8-10% and 4-5% of the total cell number, respectively. The anti-gastrin serum stained 330-360 nerve cells in the cerebral, 32-46 in the subesophageal and 7-25 in the ventral cord ganglia, representing 15-16%, 2-3% and 0.5-2% of the total cell number. Immunopositivity was found with both antisera in the enteric nervous system, where the stomatogastric ganglia and the enteric plexus contain immunoreactive cells and fibers. Immunopositive cells were found in the epithelial and subepithelial cells, as well as in nerve cells innervating the muscular layer of the gastrointestinal tube. Various epidermal sensory cells also displayed strong immunoreactivity. According to our findings and the results of several functional studies, it is suggested that in annelids cholecystokinin- and gastrin-like peptides may be involved in digestive regulation, sensory processes and central integrating processes.
利用免疫组织化学方法，研究了两种环节动物，即蚯蚓和赤子爱胜蚓神经系统中胆囊收缩素和胃泌素样免疫反应性细胞体及纤维的分布情况。脑神经节含有170 - 250个、咽下神经节含有120 - 150个、腹神经节含有50 - 75个胆囊收缩素免疫反应性细胞，分别占细胞总数的8 - 12%、8 - 10%和4 - 5%。抗胃泌素血清在脑神经节中染色330 - 360个神经细胞，在咽下神经节中染色32 - 46个，在腹神经节中染色7 - 25个，分别占细胞总数的15 - 16%、2 - 3%和0.5 - 2%。在肠神经系统中，两种抗血清均发现免疫阳性，其中口胃神经节和肠丛含有免疫反应性细胞和纤维。在胃肠道管肌层的上皮细胞、上皮下细胞以及支配这些细胞的神经细胞中均发现免疫阳性细胞。各种表皮感觉细胞也显示出强烈的免疫反应性。根据我们的研究结果以及多项功能研究的结果，提示在环节动物中，胆囊收缩素和胃泌素样肽可能参与消化调节、感觉过程和中枢整合过程。
