Lubics A, Reglödi D, Slezák S, Szelier M, Lengvári I
Department of Anatomy, University Medical School of Pécs, Hungary.
Acta Histochem. 1997 Nov;99(4):459-67. doi: 10.1016/s0065-1281(97)80037-2.
In the present study co-localization of serotonin and FMRFamide-like immunoreactivities was studied in the central nervous system of the earthworm, Eisenia fetida. Alternate serial sections were stained with antisera raised against serotonin and FMRFamide, using the peroxidase-antiperoxidase method. The immunostained consecutive sections were compared by light-microscopy and on reconstructed images using NIH Image and Neurolucida programs. Although there was a large number of serotonin and FMRFamide-like immunoreactive neurons in the central nervous system of Eisenia, co-localization of immunostaining was relatively rare. In the cerebral ganglion no cells were found that stained for both antisera. In the subesophageal and ventral cord ganglia the neurons reacting for both antisera represented approximately 17-23% and 10-14% of the serotonin- and 7-11% and 9-17% of the FMRF-amide-like immunoreactive cells, respectively. It is suggested, that FMRFamide-related peptides act as neuromodulators and may also influence the activity of serotonergic neurons.
在本研究中，对赤子爱胜蚓中枢神经系统中5-羟色胺与类FMRF酰胺免疫反应性的共定位进行了研究。使用过氧化物酶-抗过氧化物酶方法，用针对5-羟色胺和FMRF酰胺产生的抗血清对交替的连续切片进行染色。通过光学显微镜以及使用NIH Image和Neurolucida程序在重建图像上对免疫染色的连续切片进行比较。尽管在赤子爱胜蚓的中枢神经系统中有大量的5-羟色胺和类FMRF酰胺免疫反应性神经元，但免疫染色的共定位相对较少。在脑神经节中未发现对两种抗血清均呈阳性染色的细胞。在咽下神经节和腹神经索神经节中，对两种抗血清均有反应的神经元分别约占5-羟色胺免疫反应性细胞的17%-23%和FMRF酰胺样免疫反应性细胞的7%-11%以及9%-17%。有人提出，FMRF酰胺相关肽可作为神经调质，也可能影响5-羟色胺能神经元的活性。
