Rymar' S E, Isakova I A, Kirilenko S D, Kordium V A
Tsitol Genet. 1999 Mar-Apr;33(2):74-8.
The specific to 3 types of Cry genes primers containing inosine were constructed to find crystal insecticidal protein Bacillus thuringiensis genes using PCR. A number of new B. thuringiensis strains isolated in Ukraine were investigated using these PCR primers. As a results, Cry genes were found, some of them were identified and demonstrated high homology to Cry1Ba2 and Cry1Bc genes.
构建了含次黄嘌呤的针对3种Cry基因的引物，用于通过聚合酶链反应（PCR）寻找苏云金芽孢杆菌的晶体杀虫蛋白基因。使用这些PCR引物对在乌克兰分离出的许多新的苏云金芽孢杆菌菌株进行了研究。结果发现了Cry基因，其中一些被鉴定出来，并显示出与Cry1Ba2和Cry1Bc基因具有高度同源性。
