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[用于搜索和鉴定苏云金芽孢杆菌中杀虫晶体蛋白基因的含肌苷引物的构建与应用]

[The construction and use of inosine-containing primers for searching for and identifying the genes of insecticidal crystal proteins in Bacillus thuringiensis].

作者信息

Rymar' S E, Isakova I A, Kirilenko S D, Kordium V A

出版信息

Tsitol Genet. 1999 Mar-Apr;33(2):74-8.

Abstract

The specific to 3 types of Cry genes primers containing inosine were constructed to find crystal insecticidal protein Bacillus thuringiensis genes using PCR. A number of new B. thuringiensis strains isolated in Ukraine were investigated using these PCR primers. As a results, Cry genes were found, some of them were identified and demonstrated high homology to Cry1Ba2 and Cry1Bc genes.

摘要

构建了含次黄嘌呤的针对3种Cry基因的引物,用于通过聚合酶链反应(PCR)寻找苏云金芽孢杆菌的晶体杀虫蛋白基因。使用这些PCR引物对在乌克兰分离出的许多新的苏云金芽孢杆菌菌株进行了研究。结果发现了Cry基因,其中一些被鉴定出来,并显示出与Cry1Ba2和Cry1Bc基因具有高度同源性。

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