通过毒素的五个保守结构域对苏云金芽孢杆菌中cry7基因进行聚合酶链反应分析。
PCR analysis of cry7 genes in Bacillus thuringiensis by the five conserved blocks of toxins.
作者信息
Ben-Dov E, Manasherob R, Zaritsky A, Barak Z, Margalith Y
机构信息
Department of Life Sciences, Ben-Gurion University of the Negev, P.O. Box 653, Be'er-Sheva 84105, Israel.
出版信息
Curr Microbiol. 2001 Feb;42(2):96-9. doi: 10.1007/s0028403336.
PMID:11136129
Abstract
An alternative PCR analysis to screen for cry7 genes is proposed, based on the five conserved blocks of amino acids of Bacillus thuringiensis toxins and their encoding DNA sequences. A complete set of five primers was constructed, four direct and one reverse, yielding four specific amplicons. Modified profiles can identify new cry genes.
摘要
基于苏云金芽孢杆菌毒素的五个保守氨基酸块及其编码DNA序列，提出了一种用于筛选cry7基因的替代PCR分析方法。构建了一套完整的五个引物，四个正向引物和一个反向引物，产生四个特异性扩增子。改进的图谱可以鉴定新的cry基因。
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