Ben-Dov E, Manasherob R, Zaritsky A, Barak Z, Margalith Y
Department of Life Sciences, Ben-Gurion University of the Negev, P.O. Box 653, Be'er-Sheva 84105, Israel.
Curr Microbiol. 2001 Feb;42(2):96-9. doi: 10.1007/s0028403336.
An alternative PCR analysis to screen for cry7 genes is proposed, based on the five conserved blocks of amino acids of Bacillus thuringiensis toxins and their encoding DNA sequences. A complete set of five primers was constructed, four direct and one reverse, yielding four specific amplicons. Modified profiles can identify new cry genes.
基于苏云金芽孢杆菌毒素的五个保守氨基酸块及其编码DNA序列，提出了一种用于筛选cry7基因的替代PCR分析方法。构建了一套完整的五个引物，四个正向引物和一个反向引物，产生四个特异性扩增子。改进的图谱可以鉴定新的cry基因。
