Yamada R, Hayashi Y, Ueno S, Yamada S
Department of Ophthalmology, St. Marianna University School of Medicine, Kawasaki, Japan.
Nippon Ganka Gakkai Zasshi. 1999 Aug;103(8):591-6.
Exotoxin-induced uveitis in rabbit eyes was established by intravitreal inoculation of staphylococcal enterotoxin B (SEB). Association between findings of pulsed Doppler assessments and histological features were evaluated.
Eyes of rabbits were unilaterally inoculated with 50 ng/kg of intravitreal SEB. Pupil diameter, blood flow velocity in the central retinal artery in both eyes and heart rate were measured using an ultrasonic diagnostic apparatus after inoculation.
The pupil diameter and the mean blood flow velocity in central retinal arteries decreased and reached a trough 3-5 hours after the inoculation, then increased. Pulsatility index showed reciprocal fluctuations. Histological features at the early phase after inoculation revealed lymphocytic, plasmacytic, and polynuclear leukocytic infiltrations around the anterior chamber, limbal conjunctivae, ciliary body, and nerve fiber layer of the retina, followed by recovery after about 5 days. At the late phase, recurrent inflammations in the ciliary body and intravitreal space were observed in addition to invasion into the rod and cone layer.
Early and late phase uveitis was established by intravitreal SEB. It is considered that the uveitis may be caused by two different regulatory mechanisms.
通过玻璃体腔内接种葡萄球菌肠毒素B（SEB）在兔眼建立外毒素诱导的葡萄膜炎。评估脉冲多普勒评估结果与组织学特征之间的关联。
给兔眼单侧玻璃体腔内接种50 ng/kg的SEB。接种后使用超声诊断仪测量双眼瞳孔直径、视网膜中央动脉血流速度和心率。
接种后瞳孔直径和视网膜中央动脉平均血流速度降低，在3 - 5小时后达到最低点，然后升高。搏动指数呈现相反的波动。接种后早期的组织学特征显示前房、角膜缘结膜、睫状体和视网膜神经纤维层周围有淋巴细胞、浆细胞和多核白细胞浸润，约5天后恢复。晚期除了侵入视杆和视锥层外，还观察到睫状体和玻璃体内空间的反复炎症。
通过玻璃体腔内注射SEB建立了早期和晚期葡萄膜炎。认为葡萄膜炎可能由两种不同的调节机制引起。
