Farsam H, Souri E, Gharavi N
Department of Medicinal Chemistry, Faculty of Pharmacy, Tehran University of Medical Sciences, Iran.
J Pharm Pharmacol. 1999 Jul;51(7):873-5. doi: 10.1211/0022357991773096.
We have developed a stereoselective high-performance liquid chromatography technique for analytical separation of methocarbamol enantiomers. Precolumn derivatization was performed at room temperature using (-)-menthylchloroformate as a chiral reagent in the presence of pyridine as catalyst. The resulting diastereomers were separated on two Resolve C18 columns connected in series. The mobile phase was phosphate buffer (pH 7.5)-acetonitrile (50: 50, v/v) at a flow rate of 1 mL min(-1). UV detection was set at 274 nm. The optimum amount of reagent and the maximum peak intensity of the diastereomers were determined. The resolution of the diastereomers was satisfactory (alpha = 1.04) under the conditions used.
我们开发了一种用于分析分离美索巴莫对映体的立体选择性高效液相色谱技术。在室温下，以（-）-氯甲酸薄荷酯作为手性试剂，吡啶作为催化剂进行柱前衍生化。所得非对映体在两根串联的Resolve C18柱上进行分离。流动相为磷酸盐缓冲液（pH 7.5）-乙腈（50:50，v/v），流速为1 mL min⁻¹。紫外检测波长设定为274 nm。确定了试剂的最佳用量和非对映体的最大峰强度。在所使用的条件下，非对映体的分离度令人满意（α = 1.04）。
