Department of Analytical Chemistry, Faculty of Pharmacy, University of Mansoura, Mansoura 35516, Egypt.
J Fluoresc. 2011 Mar;21(2):555-61. doi: 10.1007/s10895-010-0742-x. Epub 2010 Oct 9.
A simple, sensitive and rapid spectrofluorometric method for determination of methocarbamol in pharmaceutical formulations and spiked human plasma has been developed. The proposed method is based on the measurement of the native fluorescence of methocarbamol in methanol at 313 nm after excitation at 277 nm. The relative fluorescence intensity-concentration plot was rectilinear over the range of 0.05-2.0 μg/mL, with good correlation (r=0.9999), limit of detection of 0.007 μg/ mL and a lower limit of quantification of 0.022 μg/ mL. The described method was successfully applied for the determination of methocarbamol in its tablets without interference from co-formulated drugs, such as aspirin, diclofenac, paracetamol and ibuprofen, The results obtained were in good agreement with those obtained using the official method (USP 30).The high sensitivity of the method allowed the determination of the studied drug in spiked human plasma with average percentage recovery of 99.42 ± 3.84.
建立了一种用于测定药物制剂和人血浆中三甲氧苯氨酯的简单、灵敏、快速的荧光分光光度法。该方法基于在 277nm 激发下，甲醇中三甲氧苯氨酯的固有荧光在 313nm 处的测量。在 0.05-2.0μg/ml 范围内，相对荧光强度-浓度曲线呈线性，相关性良好（r=0.9999），检测限为 0.007μg/ml，定量下限为 0.022μg/ml。该方法成功地应用于其片剂中三甲氧苯氨酯的测定，不受阿司匹林、双氯芬酸、扑热息痛和布洛芬等共配方药物的干扰。用官方方法（USP 30）得到的结果与该方法得到的结果一致。该方法的灵敏度高，允许用平均回收率为 99.42±3.84%的方法测定人血浆中研究药物的含量。
