Michelson A D, Furman M I
Center for Platelet Function Studies, Department of Pediatrics, University of Massachusetts Medical School, Worcester 01655, USA.
Curr Opin Hematol. 1999 Sep;6(5):342-8. doi: 10.1097/00062752-199909000-00012.
Whole blood flow cytometry is a powerful new laboratory technique for assessment of platelet activation and function. Flow cytometry can be used to measure platelet hyperreactivity, circulating activated platelets, leukocyte-platelet aggregates, and procoagulant platelet-derived microparticles in a number of clinical settings, including acute coronary syndromes, angioplasty, cardiopulmonary bypass, acute cerebrovascular ischemia, peripheral vascular disease, diabetes mellitus, preeclampsia, and Alzheimer's disease. Clinical applications of whole blood flow cytometric assays of platelet function in these diseases may include identification of patients who would benefit from additional antiplatelet therapy and prediction of ischemic events. Circulating monocyte-platelet aggregates appear to be a more sensitive marker of in vivo platelet activation than circulating P-selectin-positive platelets. Flow cytometry can also be used in the following clinical settings: monitoring of glycoprotein IIb-IIIa antagonist therapy, diagnosis of inherited deficiencies of platelet surface glycoproteins, diagnosis of storage pool disease, diagnosis of heparin-induced thrombocytopenia, and measurement of the rate of thrombopoiesis.
全血流式细胞术是一种用于评估血小板活化和功能的强大新实验室技术。流式细胞术可用于在多种临床环境中测量血小板高反应性、循环活化血小板、白细胞 - 血小板聚集体和促凝血血小板衍生微粒，这些临床环境包括急性冠状动脉综合征、血管成形术、体外循环、急性脑血管缺血、外周血管疾病、糖尿病、先兆子痫和阿尔茨海默病。在这些疾病中，全血流式细胞术检测血小板功能的临床应用可能包括识别可能从额外抗血小板治疗中获益的患者以及预测缺血事件。循环单核细胞 - 血小板聚集体似乎是体内血小板活化比循环P - 选择素阳性血小板更敏感的标志物。流式细胞术还可用于以下临床环境：监测糖蛋白IIb - IIIa拮抗剂治疗、诊断血小板表面糖蛋白遗传性缺陷、诊断储存池病、诊断肝素诱导的血小板减少症以及测量血小板生成率。
