Savary S, Drezen J M, Tan F, Beckage N E, Periquet G
Institut de Recherche sur la Biologie de l'Insecte-UPRESA CNRS 6035, Faculté des Sciences, Parc de Grandmont, Tours, France.
Insect Mol Biol. 1999 Aug;8(3):319-27. doi: 10.1046/j.1365-2583.1999.83130.x.
Cotesia congregata polydnavirus (CcPDV) is essential for the successful parasitism of Manduca sexta larvae by the braconid wasp Cotesia congregata. In the absence of PDV, parasitoid eggs are encapsulated. Molecular analysis has demonstrated that polydnavirus sequences are integrated in the wasp chromosomes, and an ultrastructural analysis has shown that PDV replication occurs in the calyx region in the ovaries of the wasp. The bracovirus sequences appear to be excised from the wasp genome in the calyx cells where the virus replicates. Following excision of the virus sequences, the flanking sequences are rejoined. We analysed the production of two polydnavirus circles during wasp development and in different body parts of the adults of both sexes. Our study indicates that the excision of viral sequences is developmentally regulated, beginning in the pupal stage. In the adult wasp, excision occurs ubiquitously. However, regulation in the adult seems to occur only in diploid individuals, as no excision is detected in haploid males produced from virgin females.
聚瘤姬蜂多角体病毒（CcPDV）对于茧蜂科聚瘤姬蜂成功寄生烟草天蛾幼虫至关重要。在没有多角体病毒的情况下，寄生蜂的卵会被包囊化。分子分析表明，多角体病毒序列整合在黄蜂染色体中，超微结构分析表明，多角体病毒在黄蜂卵巢的萼区进行复制。茧蜂病毒序列似乎在病毒复制的萼细胞中从黄蜂基因组中切除。病毒序列切除后，侧翼序列重新连接。我们分析了黄蜂发育过程中以及成年雌雄黄蜂不同身体部位两种多角体病毒环的产生情况。我们的研究表明，病毒序列的切除受发育调控，始于蛹期。在成年黄蜂中，切除普遍发生。然而，成年黄蜂中的调控似乎仅发生在二倍体个体中，因为从未受精雌蜂产生的单倍体雄蜂中未检测到切除现象。
