Alvarez F V, Olander J, Crimmins D, Prieto B, Paz A, Alonso R, Porter S, Hess J, Crist R D, Landt Y, Ladenson J H
Servicio de Análisis Clínicos, Hospital San Agustín, Aviles 33400 Asturias, Spain.
Clin Chem. 1999 Sep;45(9):1614-20.
Current methods for obtaining fetal cells for prenatal diagnosis are invasive and carry a small (0.5-1.0%) but definite risk of miscarriage. An attractive alternative would be isolation of fetal cells from peripheral maternal blood using antibodies with high specificity and avidity.
To generate antibodies, we purified nucleated red blood cells (NRBCs) from fetal livers and used them as the immunogen to generate monoclonal antibodies (mAbs) directed against surface antigens.
The four antibodies recognized at least two conformationally sensitive epitopes of the transferrin receptor. Isolation of NRBCs from 252 maternal blood samples using these antibodies in magnetic activated cell sorting after an initial density gradient centrifugation yielded 0-419 NRBCs per 25 mL of maternal blood. One antibody, 2B7.4, not only isolated the highest number of NRBCs (>10 in 90% of the samples) but also isolated these NRBCs in 78 consecutive maternal samples.
Antibody 2B7.4 shows promise for the isolation of NRBCs from maternal blood and should allow studies concerning the source of these cells, fetal vs maternal, and the factors controlling their prevalence.
目前用于获取胎儿细胞进行产前诊断的方法具有侵入性，会带来小概率（0.5 - 1.0%）但明确的流产风险。一种有吸引力的替代方法是使用具有高特异性和亲和力的抗体从孕妇外周血中分离胎儿细胞。
为了产生抗体，我们从胎儿肝脏中纯化有核红细胞（NRBCs），并将其用作免疫原以产生针对表面抗原的单克隆抗体（mAbs）。
这四种抗体识别转铁蛋白受体的至少两个构象敏感表位。在初始密度梯度离心后，使用这些抗体通过磁激活细胞分选从252份孕妇血样中分离NRBCs，每25 mL孕妇血中获得0 - 419个NRBCs。一种抗体2B7.4不仅分离出数量最多的NRBCs（90%的样本中>10个），而且在连续78份孕妇样本中都能分离出这些NRBCs。
抗体2B7.4在从孕妇血中分离NRBCs方面显示出前景，应该有助于开展关于这些细胞来源（胎儿与母体）以及控制其存在率的因素的研究。
