Gänshirt D, Smeets F W, Dohr A, Walde C, Steen I, Lapucci C, Falcinelli C, Sant R, Velasco M, Garritsen H S, Holzgreve W
Department of Obstetrics and Gynecology, University of Basel, Switzerland.
Fetal Diagn Ther. 1998 Sep-Oct;13(5):276-86. doi: 10.1159/000020854.
We wanted to obtain statistically relevant data about the efficiency of our method for the isolation of fetal nucleated red blood cells (NRBCs) from the maternal circulation.
More than 600 samples were investigated using a triple density gradient followed by magnetic separation of anti-CD71-labeled cells, and yields and purities of recovered NRBCs were determined.
The enrichment effectivity as well as the morphological condition of cells was reproducibly good, if blood samples were enriched within 48 h after sampling. The efficacy was independent of various methodological parameters and our technique was superior to other magnetic cell-sorting techniques. Mean yields and purities of NRBCs increased with increasing gestational age, ranging from 100 to 1,000 cells per 40-ml blood sample and from 0.1 to 1%, respectively, from the 6th week of gestation to term. In pregnancies with preeclampsia NRBCs were increased by a factor of 10.
Our enrichment technique proved to be optimized with respect to various methodological parameters, which were compared in the present study, and it is efficient and reproducible for the enrichment of NRBCs from the maternal circulation in all three gestational trimesters.
我们希望获取关于从母体循环中分离胎儿有核红细胞（NRBCs）的方法效率的具有统计学意义的数据。
使用三重密度梯度随后对抗CD71标记的细胞进行磁分离，对600多个样本进行了研究，并测定了回收的NRBCs的产量和纯度。
如果血样在采样后48小时内进行富集，细胞的富集效果以及形态状况可重复地良好。该效果与各种方法学参数无关，并且我们的技术优于其他磁性细胞分选技术。NRBCs的平均产量和纯度随着孕周增加而增加，从妊娠第6周到期末，每40毫升血样分别为100至1000个细胞和0.1%至1%。在先兆子痫的妊娠中，NRBCs增加了10倍。
就本研究中比较的各种方法学参数而言，我们的富集技术已被证明是优化的，并且对于在所有三个妊娠阶段从母体循环中富集NRBCs是有效且可重复的。
