拟南芥端粒酶逆转录酶同源物AtTERT的分子克隆与特性分析

Molecular cloning and characterization of AtTERT, a telomerase reverse transcriptase homolog in Arabidopsis thaliana.

作者信息

Oguchi K, Liu H, Tamura K, Takahashi H

机构信息

Institute of Molecular and Cellular Biosciences, The University of Tokyo, Bunkyo-ku, Tokyo, Japan.

出版信息

FEBS Lett. 1999 Sep 3;457(3):465-9. doi: 10.1016/s0014-5793(99)01083-2.

DOI:10.1016/s0014-5793(99)01083-2

PMID:10471830

Abstract

On the basis of its predicted homology to human telomerase reverse transcriptase (hTERT), a cDNA for Arabidopsis thaliana TERT (AtTERT) has now been isolated from cultured cells. The cDNA contains an open reading frame of 3372 bp, encoding a protein with a predicted size of 131 kDa and isoelectric point of 9.9. The AtTERT protein contains the conserved reverse transcriptase motifs 1, 2 and A-E as well as the TERT-specific T motif. Reverse transcription-polymerase chain reaction analysis and an assay of telomerase activity revealed that both AtTERT mRNA and telomerase activity are abundant in shoot apical meristems but are not detectable in rosette leaves.

摘要

基于其与人类端粒酶逆转录酶（hTERT）的预测同源性，现已从培养细胞中分离出拟南芥TERT（AtTERT）的cDNA。该cDNA包含一个3372 bp的开放阅读框，编码一种预测大小为131 kDa、等电点为9.9的蛋白质。AtTERT蛋白包含保守的逆转录酶基序1、2和A-E以及TERT特异性T基序。逆转录-聚合酶链反应分析和端粒酶活性测定表明，AtTERT mRNA和端粒酶活性在茎尖分生组织中丰富，但在莲座叶中未检测到。