Institute of Biophysics of the Czech Academy of Sciences, Královopolská 135, CZ-61265 Brno, Czech Republic.
Laboratory of Functional Genomics and Proteomics, NCBR, Faculty of Science, Masaryk University, Kotlářská 2, CZ-61137 Brno, Czech Republic.
Int J Mol Sci. 2021 Feb 11;22(4):1783. doi: 10.3390/ijms22041783.
The gene coding for the telomerase reverse transcriptase () is essential for the maintenance of telomeres. Previously we described the presence of three paralogs in the allotetraploid plant , while a single copy was identified in the paleopolyploid model plant . Here we examine the presence, origin and functional status of variants in allotetraploid species of diverse evolutionary ages and their parental genome donors, as well as in other diploid and polyploid plant species. A combination of experimental and in silico bottom-up analyses of gene copies in polyploids revealed various patterns of retention or loss of parental variants and divergence in their functions. RT-qPCR results confirmed the expression of all the identified variants. In representative plant and green algal genomes, our synteny analyses show that their genes were located in a conserved locus that became advantageous after the divergence of eudicots, and the gene was later translocated in several plant groups. In various diploid and polyploid species, translocation of became fixed in target loci that show ancient synapomorphy.
端粒酶逆转录酶（）的基因编码对于端粒的维持是必不可少的。此前我们描述了在异源四倍体植物中存在三个 基因的同源基因，而在古多倍体模式植物中仅鉴定出一个 基因。在这里，我们研究了在不同进化年龄的异源四倍体 物种及其亲本基因组供体中 变体的存在、起源和功能状态，以及在其他二倍体和多倍体植物物种中的存在、起源和功能状态。通过对多倍体 基因拷贝的实验和从头 Bottom-up 分析的组合，揭示了亲本 变体的保留或丢失以及功能分化的各种模式。实时定量 PCR 结果证实了所有鉴定的 变体的表达。在代表性的植物和绿藻基因组中，我们的同线性分析表明，它们的 基因位于一个保守的基因座中，在真双子叶植物的分化后变得有利，之后该基因在几个植物群中发生了易位。在各种二倍体和多倍体物种中， 的易位在显示古老同源特征的靶基因座中固定下来。
