乳酸克鲁维酵母磷酸丙糖异构酶编码基因的分离、核苷酸序列及生理相关性
Isolation, nucleotide sequence, and physiological relevance of the gene encoding triose phosphate isomerase from Kluyveromyces lactis.
作者信息
Compagno C, Boschi F, Daleffe A, Porro D, Ranzi B M
机构信息
Dipartimento di Fisiologia e Biochimica Generali, Sezione Biochimica Comparata, Università degli Studi di Milano, 20133 Milan, Italy.
出版信息
Appl Environ Microbiol. 1999 Sep;65(9):4216-9. doi: 10.1128/AEM.65.9.4216-4219.1999.
Abstract
Lack of triose phosphate isomerase activity (TIM) is of special interest because this enzyme works at an important branch point of glycolytic flux. In this paper, we report the cloning and sequencing of the Kluyveromyces lactis gene encoding TIM. Unlike Saccharomyces cerevisiae DeltaTPI1 mutants, the K. lactis mutant strain was found to be able to grow on glucose. Preliminary bioconversion experiments indicated that, like the S. cerevisiae TIM-deficient strain, the K. lactis TIM-deficient strain is able to produce glycerol with high yield.
摘要
磷酸丙糖异构酶（TIM）活性的缺失尤其引人关注，因为该酶在糖酵解通量的一个重要分支点发挥作用。在本文中，我们报道了乳酸克鲁维酵母中编码TIM的基因的克隆与测序。与酿酒酵母DeltaTPI1突变体不同，发现乳酸克鲁维酵母突变菌株能够在葡萄糖上生长。初步的生物转化实验表明，与酿酒酵母TIM缺陷菌株一样，乳酸克鲁维酵母TIM缺陷菌株能够高产甘油。
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