Bouvet O M, Pernoud S, Grimont P A
Unité des Entérobactéries, Unité INSERM 389, Institut Pasteur, 75724 Paris Cedex 15, France.
Appl Environ Microbiol. 1999 Sep;65(9):4245-57. doi: 10.1128/AEM.65.9.4245-4257.1999.
The influence of growth temperature on the ability to ferment D-sorbitol was investigated in Escherichia coli O157:H7. It was found that O157:H7 strains have a temperature-sensitive sorbitol phenotype. D-Sorbitol transport and sorbitol-6-phosphate dehydrogenase activities were expressed in sorbitol-fermenting cells grown at 30 degrees C but only at a low level at 40 degrees C. Sorbitol-positive variants able to transport D-sorbitol were easily selected at 30 degrees C from culture of Sor(-) E. coli O157:H7 strains.
研究了生长温度对大肠杆菌O157:H7发酵D-山梨醇能力的影响。发现O157:H7菌株具有温度敏感的山梨醇表型。D-山梨醇转运和山梨醇-6-磷酸脱氢酶活性在30℃生长的山梨醇发酵细胞中表达,但在40℃时仅处于低水平。能够转运D-山梨醇的山梨醇阳性变体很容易在30℃下从Sor(-)大肠杆菌O157:H7菌株培养物中筛选出来。