Honda H, Hatori K, Igarashi Y, Shimada K, Matsuno K
Department of BioEngineering, Nagaoka University of Technology, Japan.
Biophys Chem. 1999 Aug 9;80(2):139-43. doi: 10.1016/s0301-4622(99)00076-9.
An actin filament exhibits distortions longitudinally when it slides upon myosin molecules. We observed that the actin filament demonstrated contractile distortions at low ATP concentrations and protractile distortions at high concentrations. Temporal development of such distortions was identified, by tracing each of several speckled fluorescent markers attached to the actin filament. Close association of the sliding movement to the moving distortions of an actin filament suggests the presence of a unitary mechanism regulating the apparently two different modes of dynamic movement.
当肌动蛋白丝在肌球蛋白分子上滑动时，它会在纵向上表现出扭曲。我们观察到，在低ATP浓度下，肌动蛋白丝表现出收缩性扭曲，而在高浓度下则表现出伸展性扭曲。通过追踪附着在肌动蛋白丝上的几个斑点荧光标记中的每一个，确定了这种扭曲的时间发展。肌动蛋白丝的滑动运动与移动扭曲的紧密关联表明存在一种单一机制，调节这两种明显不同的动态运动模式。
