肌动蛋白丝在肌球蛋白分子上滑动时的纵向扭曲和横向波动。

Longitudinal distortions and transversal fluctuations of an actin filament sliding on Myosin molecules.

作者信息

Honda H, Kikuchi K, Hatori K, Imai E, Shimada K, Matsuno K

机构信息

Department of BioEngineering, Nagaoka University of Technology, Nagaoka, 940-2188 Japan.

出版信息

J Biol Phys. 2002 Sep;28(3):359-65. doi: 10.1023/A:1020304420132.

DOI:10.1023/A:1020304420132

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3456745/

Abstract

An actin filament sliding on myosin moleculesdemonstrates both longitudinal distortions and transversal fluctuationswith the linear dimension far exceeding the diameter of an actinmonomer. Local swaying of a single actin filament was identified byreading speckled fluorescent markers attached on the filament. Theaccuracy of reading each speckled marker was about 10.4 nm (r.m.s.).Longitudinal distortions of an actin filament at a low ATP concentrationof 20 μM were as much as 0.5 μm for the average filament lengthof 5.4 μm. The magnitude of transversal fluctuations was as much as60 nm, that was independent of the filament length. Both longitudinaldistortions and transversal fluctuations are suggested to play a pivotalrole for facilitating a smooth sliding movement of an actin filament.

摘要

肌动蛋白丝在肌球蛋白分子上滑动时，表现出纵向扭曲和横向波动，其线性尺寸远超过肌动蛋白单体的直径。通过读取附着在肌动蛋白丝上的斑点荧光标记物，识别出单根肌动蛋白丝的局部摆动。读取每个斑点标记物的精度约为10.4纳米（均方根）。在20μM的低ATP浓度下，对于平均长度为5.4μm的肌动蛋白丝，其纵向扭曲高达0.5μm。横向波动的幅度高达60纳米，且与丝的长度无关。纵向扭曲和横向波动都被认为在促进肌动蛋白丝的平滑滑动运动中起着关键作用。

相似文献

1

Longitudinal distortions and transversal fluctuations of an actin filament sliding on Myosin molecules.肌动蛋白丝在肌球蛋白分子上滑动时的纵向扭曲和横向波动。

J Biol Phys. 2002 Sep;28(3):359-65. doi: 10.1023/A:1020304420132.

2

Contractile and protractile coordination within an actin filament sliding on myosin molecules.肌动蛋白丝在肌球蛋白分子上滑动时的收缩与伸展协调。

Biophys Chem. 1999 Aug 9;80(2):139-43. doi: 10.1016/s0301-4622(99)00076-9.

3

Transition from contractile to protractile distortions occurring along an actin filament sliding on myosin molecules.沿着在肌球蛋白分子上滑动的肌动蛋白丝发生的从收缩性扭曲到伸展性扭曲的转变。

Biophys Chem. 2004 Feb 15;107(3):283-8. doi: 10.1016/j.bpc.2003.09.011.

4

Enhancing the staggered fluctuations of an actin filament sliding on Chara myosin.增强肌动蛋白丝在轮藻肌球蛋白上滑动时的交错波动。

Biophys Chem. 2004 Jun 1;109(3):345-50. doi: 10.1016/j.bpc.2003.12.007.

5

Staggered movement of an actin filament sliding on myosin molecules in the presence of ATP.在ATP存在的情况下，肌动蛋白丝在肌球蛋白分子上滑动的交错运动。

Biophys Chem. 1998 Mar 9;70(3):241-5. doi: 10.1016/s0301-4622(97)00129-4.

6

Fluctuation of local points of F-actin sliding on the surface-fixed H-meromyosin molecules in the presence of ATP.在ATP存在的情况下，F-肌动蛋白在表面固定的重酶解肌球蛋白分子上滑动的局部点的波动。

Biophys Chem. 2001 Oct 18;93(1):23-35. doi: 10.1016/s0301-4622(01)00203-4.

7

ATP-dependent fluctuations of single actin filaments in vitro.

Biophys Chem. 1996 Feb 8;58(3):267-72. doi: 10.1016/0301-4622(95)00100-x.

8

Slowly modulating fluctuations as mesoscopic distortions occurring on an actin filament.作为肌动蛋白丝上发生的介观畸变而缓慢调节的波动。

Biosystems. 2009 Apr;96(1):14-8. doi: 10.1016/j.biosystems.2008.11.003. Epub 2008 Nov 18.

9

Onset of the sliding movement of an actin filament on myosin molecules: from isotropic to anisotropic fluctuations.肌动蛋白丝在肌球蛋白分子上滑动运动的起始：从各向同性波动到各向异性波动。

Biophys Chem. 1999 Nov 15;82(1):29-33. doi: 10.1016/s0301-4622(99)00100-3.

10

Up-and-down movement of a sliding actin filament in the in vitro motility assay.体外运动分析中滑动肌动蛋白丝的上下运动。

Biosystems. 2011 Jan;103(1):79-84. doi: 10.1016/j.biosystems.2010.10.005. Epub 2010 Oct 21.

本文引用的文献

1

Muscle structure and theories of contraction.肌肉结构与收缩理论。

Prog Biophys Biophys Chem. 1957;7:255-318.

2

Single molecule nanomanipulation of biomolecules.生物分子的单分子纳米操纵

Trends Biotechnol. 2001 Jun;19(6):211-6. doi: 10.1016/s0167-7799(01)01635-3.

3

Link between the enzymatic kinetics and mechanical behavior in an actomyosin motor.肌动球蛋白马达中酶动力学与力学行为之间的联系。

Biophys J. 2001 Jan;80(1):379-97. doi: 10.1016/S0006-3495(01)76022-5.

4

Onset of the sliding movement of an actin filament on myosin molecules: from isotropic to anisotropic fluctuations.肌动蛋白丝在肌球蛋白分子上滑动运动的起始：从各向同性波动到各向异性波动。

Biophys Chem. 1999 Nov 15;82(1):29-33. doi: 10.1016/s0301-4622(99)00100-3.

5

Contractile and protractile coordination within an actin filament sliding on myosin molecules.肌动蛋白丝在肌球蛋白分子上滑动时的收缩与伸展协调。

Biophys Chem. 1999 Aug 9;80(2):139-43. doi: 10.1016/s0301-4622(99)00076-9.

6

Two heads of myosin are better than one for generating force and motion.肌球蛋白的两个头部在产生力量和运动方面比一个头部更具优势。

Proc Natl Acad Sci U S A. 1999 Apr 13;96(8):4402-7. doi: 10.1073/pnas.96.8.4402.

7

Fluctuation correlation in the sliding movement generated by protein motors in vitro.体外蛋白质马达产生的滑动运动中的涨落相关性。

Adv Exp Med Biol. 1998;453:47-51. doi: 10.1007/978-1-4684-6039-1_6.

8

Propagation of a signal coordinating force generation along an actin filament in actomyosin complexes.

Biophys Chem. 1998 Nov 16;75(2):81-5. doi: 10.1016/s0301-4622(98)00184-7.

9

Swing of the lever arm of a myosin motor at the isomerization and phosphate-release steps.肌球蛋白马达杠杆臂在异构化和磷酸释放步骤中的摆动。

Nature. 1998 Nov 26;396(6709):380-3. doi: 10.1038/24640.

10

Staggered movement of an actin filament sliding on myosin molecules in the presence of ATP.在ATP存在的情况下，肌动蛋白丝在肌球蛋白分子上滑动的交错运动。

Biophys Chem. 1998 Mar 9;70(3):241-5. doi: 10.1016/s0301-4622(97)00129-4.

文献AI研究员

20分钟写一篇综述，助力文献阅读效率提升50倍。

用中文搜PubMed

大模型驱动的PubMed中文搜索引擎

文档翻译

学术文献翻译模型，支持多种主流文档格式。