Lazzaroni J C, Germon P, Ray M C, Vianney A
Unité de Microbiologie et Génétique, CNRS-INSA-Université Lyon 1 (UMR 5577), Villeurbanne, France.
FEMS Microbiol Lett. 1999 Aug 15;177(2):191-7. doi: 10.1111/j.1574-6968.1999.tb13731.x.
The Tol proteins of Escherichia coli are involved in outer membrane stability. They are also required for the uptake of the group A colicins and the translocation of filamentous phage DNA into the cytoplasm. The tol-pal genes constitute two operons in the E. coli genome, orfltolQRA and tolBpalorf2. The TolQ TolR TolA proteins form a complex in the cytoplasmic membrane, while TolB and Pal interact near the outer membrane. Most of the amino acid residues of TolA, TolB, TolR and Pal are localized in the periplasm. Recent advances in the knowledge of interactions of Tol-Pal proteins with other envelope components, or with group A colicins, are presented, together with current hypotheses about the role of the Tol proteins in outer membrane stability.
大肠杆菌的Tol蛋白参与外膜稳定性的维持。它们也是A群大肠杆菌素摄取以及丝状噬菌体DNA转运至细胞质所必需的。tol-pal基因在大肠杆菌基因组中构成两个操纵子，即orfltolQRA和tolBpalorf2。TolQ、TolR、TolA蛋白在细胞质膜中形成复合物，而TolB和Pal在外膜附近相互作用。TolA、TolB、TolR和Pal的大多数氨基酸残基位于周质中。本文介绍了Tol-Pal蛋白与其他包膜成分或与A群大肠杆菌素相互作用的最新研究进展，以及关于Tol蛋白在外膜稳定性中作用的当前假说。
