Cramer H, Geselowitz D A, Torrence P F
Section of Biomedical Chemistry, NIDDK, NIH, Bethesda, MD 20892, USA.
Nucleosides Nucleotides. 1999 Jun-Jul;18(6-7):1523-5. doi: 10.1080/07328319908044776.
The development of a method for measuring the ability of 2-5A analogues to activate the cleavage of an oligoribonucleotide substrate by RNase L is described. This method is based on fluorescence resonance energy transfer. The method is easily performed with 96-well plates, allowing for quantitative high-throughput analyses of 2-5A analogues under different reaction conditions.
本文描述了一种用于测量2-5A类似物激活RNase L切割寡核糖核苷酸底物能力的方法的开发。该方法基于荧光共振能量转移。该方法易于在96孔板中进行，可对不同反应条件下的2-5A类似物进行定量高通量分析。
