Isaksson J, Maltseva T, Agback P, Luo X, Kumar A, Zamaratski E, Chattopadhyaya J
Department of Bioorganic Chemistry, Uppsala University, Sweden.
Nucleosides Nucleotides. 1999 Jun-Jul;18(6-7):1593-6. doi: 10.1080/07328319908044793.
The impact of intramolecular stereoelectronic effects has been examined by comparison of the solution structures of natural oligo-DNA duplex, 5'(1C2G3C4G5A6A7T8T9C10G11C12G)2(3'), and its carbocyclic-nucleotide analogues in which the pentose sugar in 2'-dA residue is replaced with its carbocyclic counterpart (i.e. 2'-deoxyaristeromycin). Based on the NMR evidences, it has been shown, that 2'-deoxyaristeromycin analog exists in a dynamic equilibrium between the two forms of duplexes, one with W-C bp and the second with Hoogsteen bp in ca 1:1 ratio at lower temperature (below 35 degrees C) and as hairpin at higher temperature (from approximately 40 degrees-60 degrees C).
通过比较天然寡聚 DNA 双链体 5'(1C2G3C4G5A6A7T8T9C10G11C12G)2(3')及其碳环核苷酸类似物（其中 2'-dA 残基中的戊糖被其碳环对应物取代，即 2'-脱氧阿瑞吡坦）的溶液结构，研究了分子内立体电子效应的影响。基于核磁共振证据表明，2'-脱氧阿瑞吡坦类似物在两种双链体形式之间存在动态平衡，一种是 W-C 碱基对，另一种是 Hoogsteen 碱基对，在较低温度（低于 35 摄氏度）下比例约为 1:1，在较高温度（约 40 至 60 摄氏度）下则为发夹结构。
