Noll B, Hackler R, Pelzer M, Pelzer S, Nusser P, Maisch B, Schaefer J R, Steinmetz A
Zentrum Innere Medizin, Abteilung Kardiologie, Klinikum der Philipps Universität Marburg, Germany.
Clin Chem Lab Med. 1999 Jun;37(6):643-8. doi: 10.1515/CCLM.1999.100.
Apolipoproteins (apo) C-I, C-II, and C-III play crucial roles in intravascular lipid metabolism. Whereas apo C-II is an obligate cofactor for lipoprotein lipase, apo C-III was shown to inhibit its action. Apo C-I can be a potent cofactor of human lecithin:cholesterol acyltransferase. Structural mutants and deficiencies of apo C-II lead to hypertriglyceridemia. A similar phenotype is associated with apo C-III mutants and is inducible by overexpression of human apo C-III in transgenic animals. No structural variant has so far been reported for apo C-I. The present paper describes a rapid semi-automated procedure for isoelectric focusing analysis of these C-apolipoproteins from whole plasma or serum and their visualization by immunofixation and silver staining. The procedure allows detection of charged variants of C-apolipoproteins. As applied to 295 patients with coronary heart disease and 85 controls, it also serves to detect deficiency syndromes of these apolipoproteins. The procedure provides reliable, easy and quick analysis of C-apolipoproteins applicable as a routine or screening procedure not restricted to specialized laboratories.
载脂蛋白（apo）C-I、C-II和C-III在血管内脂质代谢中发挥着关键作用。apo C-II是脂蛋白脂肪酶的必需辅因子，而apo C-III则被证明可抑制其作用。apo C-I可以是人类卵磷脂：胆固醇酰基转移酶的有效辅因子。apo C-II的结构突变体和缺陷会导致高甘油三酯血症。类似的表型与apo C-III突变体相关，并且可通过在转基因动物中过度表达人类apo C-III来诱导。迄今为止，尚未报道apo C-I的结构变异体。本文描述了一种快速半自动程序，用于对全血或血清中的这些C-载脂蛋白进行等电聚焦分析，并通过免疫固定和银染进行可视化。该程序可以检测C-载脂蛋白的带电变体。应用于295例冠心病患者和85例对照时，它还可用于检测这些载脂蛋白的缺乏综合征。该程序提供了对C-载脂蛋白的可靠、简便且快速的分析，可作为常规或筛查程序应用，不限于专业实验室。
