Ikeda H
Zenkokai Ikeda Hospital, Sakai.
Rinsho Byori. 1991 Aug;39(8):841-5.
The profiles of C-II apolipoprotein (apo C-II) or apo C-III were determined in ten normal full-term female neonates and ten healthy adult females, to characterize the distribution of apo C in umbilical cord blood (UCB). The profiles of the distribution of apo C were electrophoretically obtained. After agarose gel-immunofixation electrophoresis using anti-apo C-II or anti-apo C-III antisera, the immune complexes of apo C-II or apo C-III selectively remained in the gels by salting out the non-immunoreacted proteins and were stained to be measured by the densitometry. So, apo C in high density lipoprotein (HDL) were satisfactorily separated from apo C in apo B-containing lipoproteins (LPcB). The apolipoproteins levels in mg/dl +/- SD for all UCB were; apo A-I = 84.4 +/- 11.8; apo A-II = 17.8 +/- 5.9; apo B = 14.0 +/- 4.2; apo C-II = 2.4 +/- 0.4; apo C-III = 3.6 +/- 0.9; apo E = 3.7 +/- 1.2, and these values were, respectively, 62.5%, 55.3%, 15.9%, 68.6%, 48.0% and 90.2% of the values obtained in adult blood. Apo C profiles in UCB were characterized by the distributions of the majority of apo C to HDL, in the same manner as the distribution of apo E, and were similar to the apo C profiles acquired from adult patients with hypo-beta lipoproteinemias. The percentages of apo C-II and apo C-III in umbilical HDL to total apo C-II and total apo C-III were high, 90.3% and 91.8%, compared to the adult HDL levels of 52.4% and 51.5%, respectively.(ABSTRACT TRUNCATED AT 250 WORDS)
测定了10名正常足月女新生儿和10名健康成年女性的载脂蛋白C-II（apo C-II）或apo C-III谱，以描述脐血（UCB）中apo C的分布特征。通过电泳获得apo C的分布谱。使用抗apo C-II或抗apo C-III抗血清进行琼脂糖凝胶免疫固定电泳后，通过盐析非免疫反应蛋白，apo C-II或apo C-III的免疫复合物选择性地保留在凝胶中，并进行染色以通过光密度测定法进行测量。因此，高密度脂蛋白（HDL）中的apo C与含apo B的脂蛋白（LPcB）中的apo C得到了满意的分离。所有脐血中载脂蛋白水平（mg/dl ± SD）为：apo A-I = 84.4 ± 11.8；apo A-II = 17.8 ± 5.9；apo B = 14.0 ± 4.2；apo C-II = 2.4 ± 0.4；apo C-III = 3.6 ± 0.9；apo E = 3.7 ± 1.2，这些值分别为成人血液中所得值的62.5%、55.3%、15.9%、68.6%、48.0%和90.2%。脐血中的apo C谱的特征是大多数apo C分布于HDL，与apo E的分布方式相同，并且与从成人低β脂蛋白血症患者获得的apo C谱相似。脐HDL中apo C-II和apo C-III占总apo C-II和总apo C-III的百分比很高，分别为90.3%和91.8%，而成人HDL水平分别为52.4%和51.5%。（摘要截断于250字）
