Choi S Y, Kwok F, Bahn J H, Jeon S G, Ahn Y K, Yoon B H, Lee B R, Choi K S, Gao G Z
Department of Genetic Engineering, Hallym University, Chunchon, Korea.
Biofactors. 1999;10(1):35-42. doi: 10.1002/biof.5520100104.
Six monoclonal antibodies that recognize porcine brain pyridoxal kinase have been selected and designated as PK67, PK86, PK91, PK144, PK252 and PK275. A total of six monoclonal antibodies recognizing different epitopes of the enzyme were obtained, of which four inhibited the enzyme activity. When total proteins of porcine brain homogenate separated by SDS-PAGE were subjected to monoclonal antibodies, a single reactive protein band of molecular weight 39 kDa which comigrated with purified porcine pyridoxal kinase was detected. Using the anti-pyridoxal kinase antibodies as probes, the cross reactivities of brain pyridoxal kinase from human and other mammalian tissues and from avian sources were also investigated. Among human and all animal tissues tested, immunoreactive bands on Western blots appeared to have the same molecular mass of 39 kDa. These results indicate that mammalian brains contain only one major type of immunologically similar pyridoxal kinase, although some properties of the enzymes reported previously differed from one another.
已筛选出六种识别猪脑吡哆醛激酶的单克隆抗体，并将其命名为PK67、PK86、PK91、PK144、PK252和PK275。共获得六种识别该酶不同表位的单克隆抗体，其中四种抑制酶活性。当用SDS-PAGE分离的猪脑匀浆总蛋白与单克隆抗体反应时，检测到一条分子量为39 kDa的单一反应性蛋白带，其迁移率与纯化的猪吡哆醛激酶相同。以抗吡哆醛激酶抗体为探针，还研究了来自人和其他哺乳动物组织以及禽类来源的脑吡哆醛激酶的交叉反应性。在所有测试的人和动物组织中，Western印迹上的免疫反应条带似乎具有相同的39 kDa分子量。这些结果表明，哺乳动物脑仅含有一种主要类型的免疫相似的吡哆醛激酶，尽管先前报道的该酶的一些特性彼此不同。
