Choi E Y, Park S Y, Jang S H, Song M S, Cho S W, Choi S Y
Department of Genetic Engineering, College of Natural Sciences, Hallym University, Chunchon, Korea.
J Neurochem. 1995 Jan;64(1):371-7. doi: 10.1046/j.1471-4159.1995.64010371.x.
Monoclonal antibodies against bovine brain succinic semialdehyde reductase were produced and characterized. A total of nine monoclonal antibodies recognizing different epitopes of the enzyme were obtained, of which two inhibited the enzyme activity and three stained cytosol of rat spinal cord neurons as observed by indirect immunofluorescence microscopy. When unfractionated total proteins of bovine brain homogenate were separated by gel electrophoresis and immunoblotted, the antibodies specifically recognized a single protein band of 34 kDa, which comigrates with purified bovine succinic semialdehyde reductase. Using the antisuccinic semialdehyde reductase antibodies as probes, we investigated the cross-reactivities of brain succinic semialdehyde reductases from some mammalian and an avian species. The immunoreactive bands on western blots appeared to be the same in molecular mass--34 kDa--in all animal species tested, including humans. The result indicates that brain succinic semialdehyde reductase is distinct from other aldehyde reductases and that mammalian brains contain only one succinic semialdehyde reductase. Moreover, the enzymes among the species are immunologically very similar, although some properties of the enzymes reported previously were different from one another.
制备并鉴定了抗牛脑琥珀酸半醛还原酶的单克隆抗体。共获得了九种识别该酶不同表位的单克隆抗体，其中两种抑制酶活性，三种通过间接免疫荧光显微镜观察可使大鼠脊髓神经元的胞质溶胶染色。当牛脑匀浆的未分级总蛋白通过凝胶电泳分离并进行免疫印迹时，这些抗体特异性识别一条34 kDa的单一蛋白带，该蛋白带与纯化的牛琥珀酸半醛还原酶迁移率相同。使用抗琥珀酸半醛还原酶抗体作为探针，我们研究了一些哺乳动物和鸟类物种脑琥珀酸半醛还原酶的交叉反应性。在所有测试的动物物种（包括人类）中，蛋白质印迹上的免疫反应带在分子量上似乎相同——34 kDa。结果表明，脑琥珀酸半醛还原酶与其他醛还原酶不同，并且哺乳动物脑仅含有一种琥珀酸半醛还原酶。此外，尽管先前报道的该酶的一些特性彼此不同，但不同物种间的酶在免疫方面非常相似。
