Li Y, Carlson E, Murakami K, Copin J C, Luche R, Chen S F, Epstein C J, Chan P H
Department of Neurological Surgery, University of California, School of Medicine, San Francisco 94143, USA.
J Neurosci Methods. 1999 Jul 1;89(1):49-55. doi: 10.1016/s0165-0270(99)00037-0.
Copper zinc superoxide dismutase (CuZnSOD) is an important enzyme for the detoxification of reactive oxygen species. Particularly in the central nervous system (CNS), reactive oxygen species are often associated with acute brain injuries and chronic neurodegeneration. It has been demonstrated in vivo that there is an inverse correlation between CuZnSOD activity and neuronal death after acute brain injury. To further understand the protective role of CuZnSOD upon neurons, we have generated transgenic mouse lines with targeted expression of the human CuZnSOD gene (SOD1) that is driven by a rat neuron-specific enolase gene promoter in neurons of the CNS. The transgenic SOD1 expression was restricted to the CNS identified by reverse transcriptase polymerase chain reaction and SOD gel electrophoresis assays. The CuZnSOD activity was significantly increased in the brain stem of the transgenic mice. Immunostaining of human CuZnSOD activity showed that Purkinje cells in the cerebellar cortex were the most intensely stained neurons in the CNS of the transgenic mice.
铜锌超氧化物歧化酶（CuZnSOD）是一种用于清除活性氧的重要酶。特别是在中枢神经系统（CNS）中，活性氧常与急性脑损伤和慢性神经退行性变有关。体内研究表明，急性脑损伤后CuZnSOD活性与神经元死亡呈负相关。为了进一步了解CuZnSOD对神经元的保护作用，我们构建了转基因小鼠品系，其在中枢神经系统神经元中由大鼠神经元特异性烯醇化酶基因启动子驱动人CuZnSOD基因（SOD1）的靶向表达。通过逆转录聚合酶链反应和SOD凝胶电泳分析确定，转基因SOD1的表达仅限于中枢神经系统。转基因小鼠脑干中的CuZnSOD活性显著增加。人CuZnSOD活性的免疫染色显示，小脑皮质中的浦肯野细胞是转基因小鼠中枢神经系统中染色最强烈的神经元。
