三叶因子肽在角膜疾病和翼状胬肉中的分布。

Distribution of TFF peptides in corneal disease and pterygium.

作者信息

Steven P, Schäfer G, Nölle B, Hinz M, Hoffmann W, Paulsen F

机构信息

University Eye Hospital, UKSH, Campus Lübeck, Germany.

出版信息

Peptides. 2004 May;25(5):819-25. doi: 10.1016/j.peptides.2004.01.023.

DOI:10.1016/j.peptides.2004.01.023

PMID:15177877

Abstract

The central cornea of 10 cadavers and 33 patients suffering from keratoconus, herpetic keratitis, Fuchs' dystrophy and pterygium were analysed focusing on the expression of TFF peptides by means of reverse transcription polymerase chain reaction and immunohistochemistry. TFF1 and TFF3 transcripts were detected in healthy corneae as well as in pterygia. Only TFF3 mRNA was transcribed in keratoconus, Fuchs' dystrophy and herpetic keratitis. Immunohistochemistry revealed absence of all three TFF peptides in healthy corneae but production of TFF3 in each of the diseased corneae. In pterygia both TFF1 and TFF3 synthesis was detectable in goblet cells. The absence of TFF peptide production in the healthy cornea indicates that TFF3 secretion is induced in different corneal diseases by yet unknown stimuli. Here TFF3 synthesis can be interpreted as a protection mechanism, because all corneal diseases analysed are characterized by progressive tissue destruction. TFF1 and TFF3 production by goblet cells in pterygia is comparable to the healthy conjunctiva suggesting that TFF peptides do not play a significant role in the pathogenesis of pterygia.

摘要

通过逆转录聚合酶链反应和免疫组织化学方法，对10例尸体以及33例患有圆锥角膜、疱疹性角膜炎、富克斯角膜内皮营养不良和翼状胬肉患者的中央角膜进行分析，关注三叶因子（TFF）肽的表达情况。在健康角膜以及翼状胬肉中检测到了TFF1和TFF3转录本。在圆锥角膜、富克斯角膜内皮营养不良和疱疹性角膜炎中仅转录了TFF3 mRNA。免疫组织化学显示健康角膜中所有三种TFF肽均不存在，但在每种患病角膜中均有TFF3产生。在翼状胬肉中，杯状细胞中可检测到TFF1和TFF3的合成。健康角膜中不产生TFF肽表明，在不同的角膜疾病中，TFF3的分泌是由未知刺激诱导的。在此，TFF3的合成可被解释为一种保护机制，因为所分析的所有角膜疾病都以进行性组织破坏为特征。翼状胬肉中杯状细胞产生TFF1和TFF3与健康结膜相当，这表明TFF肽在翼状胬肉的发病机制中不发挥重要作用。