Kwok R, Nässel D R, Lange A B, Orchard I
Department of Zoology, University of Toronto, ON, Canada.
Peptides. 1999;20(6):687-94. doi: 10.1016/s0196-9781(99)00051-0.
The presence of locustatachykinin (LomTK)-like immunoreactivity is demonstrated in the central nervous system (CNS) of Locusta migratoria with the use of a polyclonal antiserum raised against LomTK1. By developing a radioimmunoassay with the same antiserum, we have demonstrated picomolar amounts of LomTK-like material in the tissues of the central nervous system. In contrast, only femptomolar amounts of LomTK-like material are associated with the oviduct tissue. The relative amounts of the different LomTK isoforms in the brain and the abdominal ganglionic chain were examined by separating the native peptides on high-performance liquid chromatography and comparing their retention times to synthetic LomTK standards. The amounts of the different isoforms of LomTK differed between and within the two regions of the central nervous system. However, the ratios of the different isoform amounts were similar between the two regions. The myostimulatory activities of LomTKs 1 to 4 were characterized by using the locust oviduct bioassay. LomTKs 1, 2, and 3 appeared to be more efficacious than LomTK4.
利用针对蝗虫速激肽1（LomTK1）制备的多克隆抗血清，在飞蝗的中枢神经系统（CNS）中证实了存在蝗虫速激肽（LomTK）样免疫反应性。通过使用同一抗血清开发放射免疫分析法，我们已证实在中枢神经系统组织中存在皮摩尔量的LomTK样物质。相比之下，输卵管组织中仅存在飞摩尔量的LomTK样物质。通过在高效液相色谱上分离天然肽并将它们的保留时间与合成LomTK标准品进行比较，研究了脑和腹神经节链中不同LomTK同工型的相对含量。中枢神经系统的这两个区域之间以及内部，LomTK不同同工型的含量均有所不同。然而，两个区域中不同同工型含量的比率相似。利用蝗虫输卵管生物测定法对LomTKs 1至4的肌刺激活性进行了表征。LomTKs 1、2和3似乎比LomTK4更有效。
