Muren J E, Nässel D R
Department of Zoology, Stockholm University, Sweden.
Brain Res. 1996 Nov 11;739(1-2):314-21. doi: 10.1016/s0006-8993(96)00841-4.
A radioimmunoassay was developed for insect tachykinin-related peptides with the use of an antiserum raised to the locust neuropeptide locustatachykinin I (LomTK I). Determination of tachykinin-related peptide was performed in different tissues of the cockroach Leucophaea maderae. The largest amounts of LomTK-like immunoreactivity (LomTK-LI) reside in the brain and in the midgut. Relatively large amounts were also found in the suboesophageal ganglion and throughout the ganglia of the ventral nerve cord, whereas smaller amounts of LomTK-LI were detected in the corpora cardiaca, foregut and hindgut. Extracts of unfused abdominal ganglia and midguts, respectively, were analysed by a combination of reversed phase high performance liquid chromatography, and radioimmunoassay for LomTK-LI. The extracts of abdominal ganglia and midguts both contain LomTK-LI material which separates in at least two major components. This LomTK-LI material had retention times corresponding approximately to those of synthetic LomTK I and II. Since the cellular source of LomTK-LI material in the foregut and hindgut was not known from earlier studies, we investigated these tissues by immunocytochemistry. We found that the LomTK-LI material associated with the foregut was in arborizing fibres in the oesophageal and gastric nerves and in the ingluvial ganglion. In the hindgut the muscle layer was innervated by immunoreactive fibres derived from cell bodies in the terminal ganglion. The amount of LomTK-LI material in other portions of the nervous system correlates well with previous immunocytochemical data. We conclude that L. maderae have two or more isoforms of tachykinin-related peptides in the nervous system and intestine and that these are present in various amounts in different parts of the central nervous system and intestine. The relative large amounts of LomTK-LI material in the suboesophageal ganglion, oesophageal nerve and associated ganglia and intestine indicate important roles of tachykinin-related peptides in feeding and digestion.
利用针对蝗虫神经肽蝗虫速激肽I（LomTK I）产生的抗血清，开发了一种用于昆虫速激肽相关肽的放射免疫分析法。在德国蜚蠊的不同组织中进行了速激肽相关肽的测定。最大量的LomTK样免疫反应性（LomTK-LI）存在于脑和中肠中。在咽下神经节和整个腹神经索的神经节中也发现了相对大量的LomTK-LI，而在围心腺、前肠和后肠中检测到的LomTK-LI量较少。分别对未融合的腹神经节和中肠提取物进行反相高效液相色谱和LomTK-LI放射免疫分析相结合的分析。腹神经节和中肠提取物均含有LomTK-LI物质，该物质至少分离为两个主要成分。这种LomTK-LI物质的保留时间大约与合成的LomTK I和II的保留时间相对应。由于早期研究中尚不清楚前肠和后肠中LomTK-LI物质的细胞来源，我们通过免疫细胞化学对这些组织进行了研究。我们发现，与前肠相关的LomTK-LI物质存在于食管和胃神经以及 ingluvial神经节中的分支纤维中。在后肠中，肌肉层由来自终末神经节中细胞体的免疫反应性纤维支配。神经系统其他部分的LomTK-LI物质的量与先前的免疫细胞化学数据相关性良好。我们得出结论，德国蜚蠊在神经系统和肠道中具有两种或更多种速激肽相关肽的异构体，并且这些异构体在中枢神经系统和肠道的不同部位以不同量存在。咽下神经节、食管神经以及相关神经节和肠道中相对大量的LomTK-LI物质表明速激肽相关肽在摄食和消化中起重要作用。
