Ranford-Cartwright L, Walliker D
Institute of Cell, Animal and Population Biology, University of Edinburgh, UK.
Mol Biochem Parasitol. 1999 Jul 30;102(1):13-20. doi: 10.1016/s0166-6851(99)00069-9.
We report an in situ PCR technique for visualising amplified DNA of blood forms of Plasmodium falciparum on microscope slides by fluorescence microscopy. The method is used to assess the changes in frequency of different alleles of the MSP1 gene in cultures of the progeny of a cross. We show that parasites with a recombinant form of this protein possess an initial growth advantage before declining in numbers over the long-term.
我们报告了一种原位PCR技术，可通过荧光显微镜在载玻片上观察恶性疟原虫血液形态的扩增DNA。该方法用于评估杂交后代培养物中MSP1基因不同等位基因频率的变化。我们发现，具有这种蛋白重组形式的寄生虫在数量长期下降之前具有初始生长优势。
