Takala Shannon, Branch OraLee, Escalante Ananias A, Kariuki Simon, Wootton John, Lal Altaf A
Molecular Vaccine Section, Division of Parasitic Diseases, National Center for Infectious Diseases, Centers for Disease Control and Prevention, Mail Stop-F12, 4770 Buford Hwy., Atlanta, GA 30341, USA.
Mol Biochem Parasitol. 2002 Nov-Dec;125(1-2):163-71. doi: 10.1016/s0166-6851(02)00237-2.
We have investigated intragenic recombination in Block 2 of the merozoite surface protein-1 (MSP-1), where three allele-specific families: K1, Mad20, and RO33 were previously known. Using parasites from western Kenya, we have found a fourth Block 2 allele type, which is a recombinant between Mad20 and RO33 alleles. These recombinant alleles, which we have termed MR, contain sequence from the 5' region of Mad20 and the 3' region of RO33. The results of this study provide new data on the complexity of the MSP-1 antigen gene, which is a candidate vaccine antigen, and further support the importance of intragenic recombination in generating genetic variability in Plasmodium falciparum parasites in nature.
我们研究了裂殖子表面蛋白-1（MSP-1）第2区的基因内重组情况，此前已知该区域存在三个等位基因特异性家族：K1、Mad20和RO33。利用来自肯尼亚西部的疟原虫，我们发现了第四种第2区等位基因类型，它是Mad20和RO33等位基因之间的重组体。这些我们称为MR的重组等位基因，包含Mad20 5'区域和RO33 3'区域的序列。本研究结果为作为候选疫苗抗原的MSP-1抗原基因的复杂性提供了新数据，并进一步支持了基因内重组在自然界恶性疟原虫中产生遗传变异的重要性。
