Vanhamme L, Postiaux S, Poelvoorde P, Pays E
Department of Molecular Biology, Free University of Brussels, St. Genèse, Belgium.
Mol Biochem Parasitol. 1999 Jul 30;102(1):35-42. doi: 10.1016/s0166-6851(99)00080-8.
In Trypanosoma brucei, several genes termed ESAGs for expression site-associated genes are contained within the polycistronic transcription units of the VSG gene, and their transcription is coordinately regulated during the parasite life-cycle. Since the VSG mRNA is characterized by a drastic destabilization under conditions where translation is inhibited, we examined if this post-transcriptional control also applies to the ESAG mRNAs. While the ESAG 7/6 mRNA behaved like the VSG mRNA, the ESAG 8 and ESAG 3 mRNAs did not. We ascribe this differential behaviour to the residual transcription that still occurs only in the ESAG 7/6 region of the VSG unit under conditions where this unit is down-regulated.
在布氏锥虫中，几个被称为表达位点相关基因（ESAGs）的基因包含在VSG基因的多顺反子转录单元内，并且它们的转录在寄生虫生命周期中受到协同调控。由于VSG mRNA的特征是在翻译受到抑制的条件下会急剧去稳定化，我们研究了这种转录后调控是否也适用于ESAG mRNA。虽然ESAG 7/6 mRNA的表现与VSG mRNA相似，但ESAG 8和ESAG 3 mRNA并非如此。我们将这种差异行为归因于在VSG单元下调的条件下，仍仅在VSG单元的ESAG 7/6区域发生的残余转录。
