Daï Do Thi C, Aerts D, Steinert M, Pays E
Department of Molecular Biology, University of Brussels, Rhode Saint Genèse, Belgium.
Mol Biochem Parasitol. 1991 Oct;48(2):199-210. doi: 10.1016/0166-6851(91)90115-m.
The AnTat 11.17 variant surface glycoprotein (VSG) is synthesized in both metacyclic and bloodstream forms of Trypanosoma gambiense. We have characterized the AnTat 11.17 gene, and analyzed its expression site (ES) in the bloodstream form by Southern and Northern blotting with probes from the Trypanosoma brucei AnTat 1.3A VSG ES, and by run-on transcription. The AnTat 11.17 ES is located at the end of a 700-kb chromosome. It appears to contain all the genes (ESAGs, for Expression Site-Associated Genes) present in the AnTat 1.3A VSG ES, with the possible exception of ESAG 1. Limited nucleotide sequence analysis of ESAG cDNAs from the AnTat 11.17 ES shows considerable conservation with ESAGs of T. brucei. The transcription promoter of the AnTat 11.17 VSG ES, localized by virtue of the specific accumulation of promoter-proximal transcripts which occurs following UV irradiation, was found to be at the same relative position to the first ESAG (ESAG 7) as in AnTat 1.3A.
安塔特11.17可变表面糖蛋白（VSG）在冈比亚锥虫的循环后期和血流形式中均有合成。我们已对安塔特11.17基因进行了表征，并通过用来自布氏锥虫安塔特1.3A VSG表达位点（ES）的探针进行Southern和Northern印迹分析以及进行连续转录，分析了其在血流形式中的表达位点（ES）。安塔特11.17 ES位于一条700 kb染色体的末端。它似乎包含安塔特1.3A VSG ES中存在的所有基因（表达位点相关基因，ESAGs），可能不包括ESAG 1。对来自安塔特11.17 ES的ESAG cDNA进行的有限核苷酸序列分析表明，其与布氏锥虫的ESAGs有相当程度的保守性。通过紫外线照射后启动子近端转录本的特异性积累定位的安塔特11.17 VSG ES的转录启动子，被发现与第一个ESAG（ESAG 7）的相对位置与安塔特1.3A中的相同。
