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人类B细胞对白细胞介素-13的反应取决于细胞表型以及激活方式。

The human B cell response to IL-13 is dependent on cellular phenotype as well as mode of activation.

作者信息

Ford D, Sheehan C, Girasole C, Priester R, Kouttab N, Tigges J, King T C, Luciani A, Morgan J W, Maizel A L

机构信息

Department of Pathology, Roger Williams Medical Center, Boston University School of Medicine, MA 02118, USA.

出版信息

J Immunol. 1999 Sep 15;163(6):3185-93.

Abstract

Normal mature quiescent human B lymphocytes, isolated as a function of buoyant density, require activation for up-regulation of IL-13R constituents. Cell activation through a combination of surface Ig and CD40 receptor ligation leads to the most substantial message production for IL-13Ralpha1. Functional consequences of this receptor variation, in initially quiescent cells, includes demonstrable effects on cellular proliferation in response to ligand exposure. Variations in the method of surface activation, with particular emphasis on the CD40 receptor, reveals that immobilized CD40 ligand may be sufficient, in and of itself, to up-regulate IL-13Ralpha1, which may bear significance for B-lymphocyte bystander proliferation. Regulation of the IL-13Ralpha1 protein and message also differs as a function of cellular phenotype. Although values are greater in memory than naive B cells, as they are initially isolated from extirpated tonsils, variations in the magnitude of message and protein, as a function of surface stimulation, are more substantial in the naive subset. The magnitude of variation in message production in naive cells is associated with a more vigorous proliferative response to IL-13 than seen in memory lymphocytes. The cellular response to IL-13, as a function of activation and phenotype, is the converse of that demonstrated for IL-2. Evaluation of proliferation, receptor message, ligand binding protein production, and the response to putatively synergistic cytokines reveals that IL-2 is the predominant lymphokine utilized by memory cells. This is in contradistinction to IL-13, which along with IL-4, are the predominant moieties for naive lymphocytes.

摘要

通过浮力密度分离得到的正常成熟静止人B淋巴细胞,需要激活才能上调IL-13R成分。通过表面Ig和CD40受体连接的组合进行细胞激活,可导致IL-13Rα1产生最大量的信息。在最初静止的细胞中,这种受体变化的功能后果包括对配体暴露后的细胞增殖产生明显影响。表面激活方法的变化,特别是对CD40受体的强调,表明固定化的CD40配体本身可能足以上调IL-13Rα1,这可能对B淋巴细胞旁观者增殖具有重要意义。IL-13Rα1蛋白和信息的调节也因细胞表型而异。虽然从切除的扁桃体中最初分离出的记忆B细胞中的值高于幼稚B细胞,但作为表面刺激的函数,信息和蛋白的变化幅度在幼稚亚群中更为显著。幼稚细胞中信息产生的变化幅度与对IL-13的增殖反应比记忆淋巴细胞中更强烈有关。细胞对IL-13的反应,作为激活和表型的函数,与IL-2所显示的相反。对增殖、受体信息、配体结合蛋白产生以及对假定协同细胞因子的反应的评估表明,IL-~2是记忆细胞利用的主要淋巴因子。这与IL-13相反,IL-13与IL-4一起是幼稚淋巴细胞的主要部分。

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