Yoshizaki H, Izumi Y, Hirayama C, Fujimoto A, Kandori H, Sugitani T, Ooshima Y
Drug Safety Research Labs., Hikari Branch, Takeda Chemical Industries, Ltd., Yamaguchi, Japan.
J Toxicol Sci. 1999 Aug;24(3):199-208. doi: 10.2131/jts.24.3_199.
Sperm morphological examination, computer-assisted sperm analysis (CASA) and histopathologic examination of the testis and epididymis were performed for male rats treated orally with boric acid for 3 weeks at dosage levels of 50, 150 and 500 mg/kg/day, and treated males were mated with untreated females. None of the males treated with 500 mg/kg/day could impregnate untreated females. The fertility index showed a tendency to decrease in males treated with 150 mg/kg/day. At necropsy, the pre-implantation loss rate in females mated with males treated with 150 mg/kg/day was higher than the control values. Upon epididymal sperm analysis using the CASA system, all parameters including the number of sperm and sperm motions were found to be affected in males treated with 500 mg/kg/day, and the number of sperm, percent motile, velocities and amplitude of lateral head displacement (ALH) were affected in males treated with 150 mg/kg/day. Upon sperm morphological examination, head and tail abnormalities were observed in males treated with 150 and 500 mg/kg/day. In the histopathological examination, atrophy of the seminiferous tubules and multinucleated giant cells in the testes were observed in males treated with 500 mg/kg/day.
对口服硼酸3周、剂量分别为50、150和500毫克/千克/天的雄性大鼠进行了精子形态学检查、计算机辅助精子分析（CASA）以及睾丸和附睾的组织病理学检查，并将处理过的雄性大鼠与未处理的雌性大鼠交配。接受500毫克/千克/天剂量处理的雄性大鼠均无法使未处理的雌性大鼠受孕。生育指数显示，接受150毫克/千克/天剂量处理的雄性大鼠有下降趋势。尸检时，与接受150毫克/千克/天剂量处理的雄性大鼠交配的雌性大鼠的植入前损失率高于对照值。使用CASA系统对附睾精子进行分析时，发现接受500毫克/千克/天剂量处理的雄性大鼠的所有参数（包括精子数量和精子运动）均受到影响，而接受150毫克/千克/天剂量处理的雄性大鼠的精子数量、活动百分比、速度和侧头位移幅度（ALH）均受到影响。在精子形态学检查中，接受150和500毫克/千克/天剂量处理的雄性大鼠出现头部和尾部异常。在组织病理学检查中，接受500毫克/千克/天剂量处理的雄性大鼠的睾丸生精小管萎缩和出现多核巨细胞。
