Hirano T, Sato T, Okawa K, Kanda K, Yaegashi K, Enei H
Iwate Biotechnology Research Center, Japan.
Biosci Biotechnol Biochem. 1999 Jul;63(7):1223-7. doi: 10.1271/bbb.63.1223.
The glyceraldehyde-3-phosphate dehydrogenase (GPD) gene of Lentinus edodes was isolated from a genomic DNA library and cDNA corresponding to this gene was isolated from a mycelium cDNA library. The L. edodes GPD gene was found to encode a 337-aa protein. By comparison of the cDNA and genomic DNA sequences, the presence of eight introns in the GPD gene was confirmed. The putative amino acid sequence of the L. edodes GPD gene product showed high similarity to those of other basidiomycetes. The results of Southern blot analyses suggested that only one copy of the GPD gene is present in the genome of L. edodes. The promoter region was found to contain a CT-rich stretch, two CAAT boxes and a consensus TATA box. In addition, the transcript of the GPD gene was found to be expressed constitutively and strongly. These results suggest that the promoter of the L. edodes GPD gene may be very useful as a component of transformation vectors.
从香菇基因组DNA文库中分离出香菇甘油醛-3-磷酸脱氢酶（GPD）基因，并从菌丝体cDNA文库中分离出与该基因对应的cDNA。发现香菇GPD基因编码一种337个氨基酸的蛋白质。通过比较cDNA和基因组DNA序列，证实了GPD基因中存在8个内含子。香菇GPD基因产物的推定氨基酸序列与其他担子菌的氨基酸序列具有高度相似性。Southern印迹分析结果表明，香菇基因组中仅存在一个GPD基因拷贝。发现启动子区域含有富含CT的片段、两个CAAT框和一个共有TATA框。此外，发现GPD基因的转录本组成型且强烈表达。这些结果表明，香菇GPD基因的启动子作为转化载体的一个组成部分可能非常有用。
