Ferrarese C, Mascarucci P, Zoia C, Cavarretta R, Frigo M, Begni B, Sarinella F, Frattola L, De Simoni M G
Department of Neurology, University of Milan, Ospedale San Gerardo, Monza, Italy.
J Cereb Blood Flow Metab. 1999 Sep;19(9):1004-9. doi: 10.1097/00004647-199909000-00008.
Cytokines interleukin (IL)-6 and tumor necrosis factor (TNF)-alpha can play pathogenetic or protective roles in stroke. They are increased in the brain after experimental ischemia and in the CSF of patients with stroke. However, their presence in the periphery is still controversial. To determine the source and time-course of cytokines in blood of stroke patients, IL-6 and TNF-alpha release from blood cells and serum levels were determined in 40 patients on days 1 through 2, 4, 10, 30, and 90 after stroke. Twenty healthy age-matched volunteers were used as controls. IL-6 and TNF-alpha release from stimulated blood cells was increased in stroke patients, compared to controls. A peak response (+224%) was observed at day 4 for IL-6, while TNF-alpha release was largely and significantly increased (about three-fold compared to controls) from day 1 to 2 until day 90 after stroke. The increase in IL-6 release was significantly higher in ischemic, compared to hemorrhagic strokes, at days 1 and 4. Circulating IL-6 was increased at each time point. The ischemic processes in the CNS induces a long-lasting activation of IL-6 and TNF-alpha production in peripheral blood cells, which are a major source of serum cytokines after stroke.
细胞因子白细胞介素（IL）-6和肿瘤坏死因子（TNF）-α在中风中可发挥致病或保护作用。实验性缺血后大脑中以及中风患者的脑脊液中它们的水平会升高。然而，它们在外周血中的存在情况仍存在争议。为了确定中风患者血液中细胞因子的来源和时间进程，在中风后第1至2天、第4天、第10天、第30天和第90天对40例患者的血细胞释放IL-6和TNF-α以及血清水平进行了测定。20名年龄匹配的健康志愿者作为对照。与对照组相比，中风患者受刺激血细胞释放的IL-6和TNF-α增加。IL-6在第4天观察到峰值反应（+224%），而中风后第1至2天直至第90天，TNF-α的释放大幅且显著增加（与对照组相比约为三倍）。在第1天和第4天，缺血性中风患者IL-6释放的增加显著高于出血性中风患者。每个时间点循环中的IL-6均增加。中枢神经系统的缺血过程在外周血细胞中诱导了IL-6和TNF-α产生的长期激活，外周血细胞是中风后血清细胞因子的主要来源。
