Boydston-White S, Gopen T, Houser S, Bargonetti J, Diem M
Department of Chemistry, City University of New York, Hunter College, New York 10021, USA.
Biospectroscopy. 1999;5(4):219-27. doi: 10.1002/(SICI)1520-6343(1999)5:4<219::AID-BSPY2>3.0.CO;2-O.
Infrared spectra of myeloid leukemia (ML-1) cells are reported for cells derived from an asynchronous, exponentially growing culture, as well as for cells that were fractionated according to their stage within the cell division cycle. The observed results suggest that the cells' DNA is detectable by infrared spectroscopy mainly when the cell is in the S phase, during the replication of DNA. In the G1 and G2 phases, the DNA is so tightly packed in the nucleus that it appears opaque to infrared radiation. Consequently, the nucleic acid spectral contributions in the G1 and G2 phases would be mostly that of cytoplasmic RNA. These results suggest that infrared spectral changes observed earlier between normal and abnormal cells may have been due to different distributions of cells within the stages of the cell division cycle.
本文报道了髓系白血病（ML-1）细胞的红外光谱，这些细胞来源于异步指数生长培养物，以及根据细胞分裂周期内的阶段进行分离的细胞。观察结果表明，红外光谱主要在细胞处于S期（DNA复制期间）时可检测到细胞的DNA。在G1期和G2期，DNA在细胞核中紧密堆积，以至于对红外辐射显得不透明。因此，G1期和G2期核酸光谱的贡献主要是细胞质RNA的贡献。这些结果表明，早期观察到的正常细胞和异常细胞之间的红外光谱变化可能是由于细胞在细胞分裂周期各阶段的不同分布所致。
