Department of Physics, Faculty of Natural Sciences, Ariel University, Ariel, Israel.
School of Physics and Astronomy, Sackler Faculty of Exact Sciences, Tel Aviv University, Tel Aviv, Israel.
Sci Rep. 2017 Jun 29;7(1):4381. doi: 10.1038/s41598-017-04678-6.
The vast majority of cancer related deaths are caused by metastatic tumors. Therefore, identifying the metastatic potential of cancer cells is of great importance both for prognosis and for determining the correct treatment. Infrared (IR) spectroscopy of biological cells is an evolving research area, whose main aim is to find the spectral differences between diseased and healthy cells. In the present study, we demonstrate that Attenuated Total Reflection Fourier Transform IR (ATR-FTIR) spectroscopy may be used to determine the metastatic potential of cancer cells. Using the ATR-FTIR spectroscopy, we can identify spectral alterations that are a result of hydration or molecular changes. We examined two murine melanoma cells with a common genetic background but a different metastatic level, and similarly, two human melanoma cells. Our findings revealed that higher metastatic potential correlates with membrane hydration level. Measuring the spectral properties of the cells allows us to determine the membrane hydration levels. Thus, ATR-FTIR spectroscopy has the potential to help in cancer metastasis prognosis.
绝大多数与癌症相关的死亡都是由转移性肿瘤引起的。因此，识别癌细胞的转移潜力对于预后和确定正确的治疗方法都非常重要。生物细胞的中红外（IR）光谱学是一个不断发展的研究领域，其主要目的是找到疾病细胞和健康细胞之间的光谱差异。在本研究中，我们证明衰减全反射傅里叶变换红外（ATR-FTIR）光谱学可用于确定癌细胞的转移潜力。通过使用 ATR-FTIR 光谱学，我们可以识别因水合或分子变化而导致的光谱变化。我们检查了两个具有相同遗传背景但转移水平不同的鼠黑色素瘤细胞，以及两个人类黑色素瘤细胞。我们的研究结果表明，较高的转移潜力与膜水合水平相关。测量细胞的光谱特性可以帮助我们确定膜水合水平。因此，ATR-FTIR 光谱学有可能有助于癌症转移的预后。
