Phillips J K, Hill C E
Division of Neuroscience, John Curtin School of Medical Research, Australian National University, Canberra, ACT.
Int J Dev Neurosci. 1999 Jul;17(4):377-86. doi: 10.1016/s0736-5748(99)00032-5.
Studies in autonomic targets have shown that nerves may be required for the development and maintenance of postsynaptic receptor populations. We have examined this relationship in the rat mesenteric artery, assessing mRNA expression levels for a range of neuroreceptors after neonatal sympathectomy, using 6-hydroxydopamine or antisera directed against nerve growth factor, and sensory denervation, using capsaicin. Total RNA was extracted from 28 day old rats and reverse transcription-polymerase chain reaction was performed, using primers specific for the alpha1(A,B,D)- and alpha2(A,B,C)-adrenergic, neurokinin (NKI-NK3), muscarinic (M1-M5) and P2X purinergic (P2x1-7) receptor families. Results showed no decreases in mRNA expression of any of the specific receptor subtypes after either sympathetic or sensory denervation. Small increases in mRNA expression were detected following sensory denervation for some of the receptor subtypes. We conclude that neither sympathetic nor sensory nerves are mandatory for the expression of mRNA of a range of neuroreceptors in the mesenteric vascular bed of the rat.
对自主神经靶标的研究表明，神经可能是突触后受体群体发育和维持所必需的。我们在大鼠肠系膜动脉中研究了这种关系，在新生大鼠进行交感神经切除后，使用6-羟基多巴胺或抗神经生长因子抗血清评估一系列神经受体的mRNA表达水平，以及在进行感觉神经去支配时，使用辣椒素。从28日龄大鼠中提取总RNA，并使用针对α1(A、B、D)-和α2(A、B、C)-肾上腺素能、神经激肽（NKI-NK3）、毒蕈碱（M1-M5）和P2X嘌呤能（P2x1-7）受体家族的特异性引物进行逆转录-聚合酶链反应。结果显示，在交感神经或感觉神经去支配后，任何特定受体亚型的mRNA表达均未降低。在某些受体亚型的感觉神经去支配后，检测到mRNA表达有小幅增加。我们得出结论，在大鼠肠系膜血管床中，交感神经和感觉神经对于一系列神经受体mRNA的表达都不是必需的。
