Phillips J K, Vidovic M, Hill C E
Division of Neuroscience, John Curtin School of Medical Research, Australian National University, Canberra, Australia.
J Auton Nerv Syst. 1997 Jan 12;62(1-2):85-93. doi: 10.1016/s0165-1838(96)00114-2.
Different mechanisms mediate constriction and dilation in different vascular beds. We have used reverse transcription-polymerase chain reaction to investigate whether specific patterns of receptor gene expression may underlie these variable responses. Total RNA, from the basilar, pulmonary, mesenteric and tail arteries of anaesthetised adult Wistar rats, was reverse transcribed and amplified using primers specific for the molecular subtypes of the alpha 1(A, B, D)- and alpha 2(A, B, C)-adrenergic, neurokinin (NK1-NK3) and muscarinic (m1-m5), receptors. Results showed that the pattern of gene expression was variable with no two arteries having the same receptor profile. Messenger RNA for the alpha 1A, alpha 1B, alpha 2B, NK1, NK3, m3 and m5 receptor subtypes were detected in all vessels studied while the remaining subtypes showed a variable expression amongst the arteries. This is the first description of mRNA for the m5 muscarinic receptor in peripheral tissue. The NK3 receptor was the major neurokinin receptor expressed in all vessels except the pulmonary artery, in which the NK1 receptor was also strongly expressed. We conclude that each artery expressed a specific receptor array which may permit some unique neural and hormonal controls.
不同的机制介导不同血管床的收缩和舒张。我们利用逆转录 - 聚合酶链反应来研究受体基因表达的特定模式是否可能是这些可变反应的基础。从麻醉的成年Wistar大鼠的基底动脉、肺动脉、肠系膜动脉和尾动脉中提取总RNA，使用针对α1（A、B、D） - 和α2（A、B、C） - 肾上腺素能、神经激肽（NK1 - NK3）和毒蕈碱（m1 - m5）受体分子亚型的特异性引物进行逆转录和扩增。结果表明，基因表达模式是可变的，没有两条动脉具有相同的受体谱。在所研究的所有血管中均检测到α1A、α1B、α2B、NK1、NK3、m3和m5受体亚型的信使RNA，而其余亚型在各动脉中表现出可变表达。这是外周组织中m5毒蕈碱受体mRNA的首次描述。NK3受体是除肺动脉外所有血管中表达的主要神经激肽受体，在肺动脉中NK1受体也强烈表达。我们得出结论，每条动脉都表达特定的受体组合，这可能允许一些独特的神经和激素控制。
