Acevedo-Whitehouse K A, Constantino-Casas F, Aurioles-Gamboa D, Rodríguez-Martínez H A, Godínez-Reyes C R
Departamento de Etología, Facultad de Medicina Veterinaria y Zootecnia, Universidad Nacional Autónoma de México.
J Wildl Dis. 1999 Jul;35(3):565-8. doi: 10.7589/0090-3558-35.3.565.
A primary hepatic carcinoma with a neuroendocrine pattern was detected in an adult female California sea lion (Zalophus californianus) found dead on Granito Island in the Gulf of California (Mexico) in January 1996. At necropsy, several light yellow nodules of different sizes were observed on the entire surface of the liver and spleen. Microscopic examination of these nodules using routine haematoxylin-eosin stain, revealed cubic, polyhedral and pleomorphic cells with three to four bizarre mitotic figures per field (40X). An immunohistochemistry test revealed a positive reaction of indirect immunoperoxide to cytokeratin (CK2). This is the first known case of a primary hepatic carcinoma in free-ranging California sea lions from Mexican waters.
1996年1月，在墨西哥加利福尼亚湾的格拉尼托岛发现一只成年雌性加利福尼亚海狮（Zalophus californianus）死亡，检测发现其患有具有神经内分泌特征的原发性肝癌。尸检时，在肝脏和脾脏的整个表面观察到几个大小不同的浅黄色结节。使用常规苏木精-伊红染色对这些结节进行显微镜检查，发现细胞呈立方形、多面体形且形态多样，每个视野（40倍）有三到四个奇异的有丝分裂象。免疫组织化学检测显示间接免疫过氧化物对细胞角蛋白（CK2）呈阳性反应。这是墨西哥海域野生加利福尼亚海狮原发性肝癌的首例已知病例。
