Permi P, Heikkinen S, Kilpeläinen I, Annila A
Institute of Biotechnology, University of Helsinki, Helsinki, FIN-00014, Finland.
J Magn Reson. 1999 Sep;140(1):32-40. doi: 10.1006/jmre.1999.1817.
A method for the measurement of (1)J(NC') and (2)J(H(N))(C') coupling constants from a simplified two-dimensional [(15)N, (1)H] correlation spectrum is presented. The multiplet components of the (1)J(NC') doublet in the indirect dimension and (2)J(H(N))(C') in the direct dimension are separated into two subspectra by spin-state-selective filters. Thus each subspectrum contains no more peaks than the conventional [(15)N, (1)H]-HSQC spectrum. Furthermore, the method for the measurement of (1)J(NC') and (2)J(H(N))(C') is designed to exploit destructive relaxation interference (TROSY). The results are verified against the measurements of (1)J(NC') from spin-state-selective [(13)C', (1)H] correlation spectra recorded with additional sequence described here.
本文提出了一种从简化的二维[(15)N, (1)H]相关谱测量(1)J(NC')和(2)J(H(N))(C')耦合常数的方法。间接维度中(1)J(NC')双峰的多重峰成分和直接维度中(2)J(H(N))(C')的多重峰成分通过自旋态选择滤波器被分离成两个子谱。因此，每个子谱包含的峰数不超过传统[(15)N, (1)H]-HSQC谱。此外，测量(1)J(NC')和(2)J(H(N))(C')的方法旨在利用破坏性弛豫干扰(TROSY)。通过使用此处描述的附加序列记录的自旋态选择[(13)C', (1)H]相关谱对(1)J(NC')的测量结果进行了验证。
