Permi P, Heikkinen S, Kilpeläinen I, Annila A
Institute of Biotechnology, University of Helsinki, Helsinki, FIN-00014, Finland.
J Magn Reson. 1999 Aug;139(2):273-80. doi: 10.1006/jmre.1999.1775.
(1)H-detected two-dimensional double-/zero-quantum experiments are described for measurement of homonuclear (2)J(HH)-couplings of NH(2) or CH(2) groups in proteins. These experiments utilize multiple-quantum coherence for determination of the size and the absolute sign of the geminal scalar and dipolar couplings in the presence of broad lines. Spectra are simplified by gradient selection and spin-state selective filters.
（1）描述了用于测量蛋白质中NH₂或CH₂基团同核（2）J（HH）耦合的氢检测二维双量子/零量子实验。这些实验利用多量子相干来确定在宽谱线存在下偕偶标量和偶极耦合的大小和绝对符号。通过梯度选择和自旋态选择滤波器简化光谱。
