McSharry J J
Albany Medical College, NY, USA.
Antiviral Res. 1999 Aug;43(1):1-21. doi: 10.1016/s0166-3542(99)00039-x.
This review describes the procedures for the use of fluorochrome labeled monoclonal antibodies and flow cytometry for the detection and quantification of virus infected cells. The application of this technology for (1) identifying virus infected cells in clinical specimens obtained from human cytomegalovirus (HCMV) and human immunodeficiency virus (HIV) infected individuals; (2) screening antiviral compounds active against HCMV, HDSV and HIV; and (3) performing drug susceptibility testing for HCMV, HSV and HIV clinical isolates are reviewed. The flow cytometry drug susceptibility assay is rapid, quantitative, and easily performed. It should be considered by anyone interested in performing drug susceptibility testing for any virus for which there are reliable monoclonal antibodies.
本综述描述了使用荧光染料标记的单克隆抗体和流式细胞术检测和定量病毒感染细胞的程序。综述了该技术在以下方面的应用：（1）在从人类巨细胞病毒（HCMV）和人类免疫缺陷病毒（HIV）感染个体获得的临床标本中鉴定病毒感染细胞；（2）筛选对HCMV、HDSV和HIV有活性的抗病毒化合物；（3）对HCMV、HSV和HIV临床分离株进行药物敏感性测试。流式细胞术药物敏感性测定快速、定量且易于操作。任何有兴趣对有可靠单克隆抗体的任何病毒进行药物敏感性测试的人都应考虑采用该方法。
