使用单一单克隆抗体来确定1型和2型单纯疱疹病毒临床分离株对阿昔洛韦的敏感性。
Use of a single monoclonal antibody to determine the susceptibilities of herpes simplex virus type 1 and type 2 clinical isolates to acyclovir.
作者信息
Chutkowski Christine, Olson Betty, McDonough Ann, Mahoney James, McSharry James J
机构信息
Center for Immunology and Microbial Disease, Albany Medical Center, Albany, New York 12208, USA.
出版信息
Clin Diagn Lab Immunol. 2002 Nov;9(6):1379-81. doi: 10.1128/cdli.9.6.1379-1381.2002.
Abstract
This report describes a flow cytometry drug susceptibility assay that uses a single fluorochrome-labeled monoclonal antibody to determine the acyclovir susceptibilities of herpes simplex virus (HSV) type 1 or type 2 clinical isolates. This assay yields 50% effective doses (drug concentrations that reduce the number of antigen-positive cells by 50%) for HSV clinical isolates that are equivalent to those obtained with the plaque reduction assay.
摘要
本报告描述了一种流式细胞术药物敏感性检测方法，该方法使用单一荧光染料标记的单克隆抗体来确定1型或2型单纯疱疹病毒（HSV）临床分离株对阿昔洛韦的敏感性。对于HSV临床分离株，该检测方法得出的50%有效剂量（使抗原阳性细胞数量减少50%的药物浓度）与蚀斑减少检测法得出的结果相当。
相似文献
1
Use of a single monoclonal antibody to determine the susceptibilities of herpes simplex virus type 1 and type 2 clinical isolates to acyclovir.使用单一单克隆抗体来确定1型和2型单纯疱疹病毒临床分离株对阿昔洛韦的敏感性。
Clin Diagn Lab Immunol. 2002 Nov;9(6):1379-81. doi: 10.1128/cdli.9.6.1379-1381.2002.
2
Cytopathic effect inhibition assay for determining the in-vitro susceptibility of herpes simplex virus to antiviral agents.用于确定单纯疱疹病毒对抗病毒药物体外敏感性的细胞病变效应抑制试验。
J Antimicrob Chemother. 1999 Nov;44(5):705-8. doi: 10.1093/jac/44.5.705.
3
Comparison of a DNA probe assay with the plaque reduction assay for measuring the sensitivity of herpes simplex virus and varicella-zoster virus to penciclovir and acyclovir.用于测量单纯疱疹病毒和水痘-带状疱疹病毒对喷昔洛韦和阿昔洛韦敏感性的DNA探针检测法与蚀斑减少检测法的比较。
J Virol Methods. 1996 Jan;56(1):3-11. doi: 10.1016/0166-0934(95)01889-1.
4
A rapid screen test for in vitro susceptibility of clinical herpes simplex virus isolates.一种用于临床单纯疱疹病毒分离株体外敏感性的快速筛查试验。
J Infect Dis. 1994 Apr;169(4):879-82. doi: 10.1093/infdis/169.4.879.
5
Rapid screening tests for determining in vitro susceptibility of herpes simplex virus clinical isolates.用于确定单纯疱疹病毒临床分离株体外敏感性的快速筛查试验。
J Clin Microbiol. 1998 Aug;36(8):2389-91. doi: 10.1128/JCM.36.8.2389-2391.1998.
6
Activity of penciclovir in antiviral assays against herpes simplex virus.喷昔洛韦在抗单纯疱疹病毒抗病毒试验中的活性。
J Antimicrob Chemother. 1996 Feb;37(2):303-13. doi: 10.1093/jac/37.2.303.
7
Rapid determination of antiviral drug susceptibility of herpes simplex virus types 1 and 2 by real-time PCR.通过实时聚合酶链反应快速测定1型和2型单纯疱疹病毒的抗病毒药物敏感性。
Antiviral Res. 2006 Mar;69(3):152-7. doi: 10.1016/j.antiviral.2005.11.004. Epub 2005 Dec 19.
8
Simultaneous titration and phenotypic antiviral drug susceptibility testing for herpes simplex virus 1 and 2.单纯疱疹病毒 1 和 2 的同时滴定和表型抗病毒药物敏感性测试。
J Clin Virol. 2014 Nov;61(3):382-6. doi: 10.1016/j.jcv.2014.08.015. Epub 2014 Aug 28.
9
Three-dimensional analysis of combination effect of ellagitannins and acyclovir on herpes simplex virus types 1 and 2.鞣花单宁与阿昔洛韦联合作用对单纯疱疹病毒 1 型和 2 型的三维分析
Antiviral Res. 2011 Feb;89(2):174-81. doi: 10.1016/j.antiviral.2010.11.014. Epub 2010 Dec 4.
10
A sensitive assay system screening antiviral compounds against herpes simplex virus type 1 and type 2.一种用于筛选抗1型和2型单纯疱疹病毒的抗病毒化合物的灵敏检测系统。
J Virol Methods. 1994 Sep;49(2):169-78. doi: 10.1016/0166-0934(94)90041-8.
引用本文的文献
1
Antiviral activities of extracts from Hong Kong seaweeds.香港海藻提取物的抗病毒活性。
J Zhejiang Univ Sci B. 2008 Dec;9(12):969-76. doi: 10.1631/jzus.B0820154.
2
Phenotypic drug susceptibility assay for influenza virus neuraminidase inhibitors.流感病毒神经氨酸酶抑制剂的表型药物敏感性测定
Clin Diagn Lab Immunol. 2004 Jan;11(1):21-8. doi: 10.1128/cdli.11.1.21-28.2004.
本文引用的文献
1
Incidence of herpes simplex virus type 2 infection in the United States.美国2型单纯疱疹病毒感染的发病率。
Am J Epidemiol. 2001 May 1;153(9):912-20. doi: 10.1093/aje/153.9.912.
2
Characterization of an acyclovir-resistant herpes simplex virus type 2 strain isolated from a premature neonate.从一名早产新生儿分离出的阿昔洛韦耐药性单纯疱疹病毒2型毒株的特征分析。
J Infect Dis. 2000 Apr;181(4):1458-61. doi: 10.1086/315387. Epub 2000 Apr 13.
3
Antiviral drug susceptibility assays: going with the flow.抗病毒药物敏感性检测：顺应潮流
Antiviral Res. 1999 Aug;43(1):1-21. doi: 10.1016/s0166-3542(99)00039-x.
4
Treatment of mucocutaneous herpes simplex virus infections unresponsive to acyclovir with topical foscarnet cream in AIDS patients: a phase I/II study.艾滋病患者中对阿昔洛韦无反应的黏膜皮肤单纯疱疹病毒感染采用局部膦甲酸钠乳膏治疗：一项Ⅰ/Ⅱ期研究。
J Acquir Immune Defic Syndr. 1999 Aug 1;21(4):301-6. doi: 10.1097/00126334-199908010-00007.
5
New developments in the epidemiology, natural history and management of genital herpes.生殖器疱疹的流行病学、自然史及治疗的新进展。
Antiviral Res. 1999 May;42(1):1-14. doi: 10.1016/s0166-3542(99)00004-2.
6
Acyclovir-resistant herpes simplex virus infections in a bone marrow transplant population.骨髓移植人群中对阿昔洛韦耐药的单纯疱疹病毒感染
Bone Marrow Transplant. 1998 Sep;22(6):587-9. doi: 10.1038/sj.bmt.1701392.
7
Rapid ganciclovir susceptibility assay using flow cytometry for human cytomegalovirus clinical isolates.使用流式细胞术对人巨细胞病毒临床分离株进行更昔洛韦快速药敏试验。
Antimicrob Agents Chemother. 1998 Sep;42(9):2326-31. doi: 10.1128/AAC.42.9.2326.
8
Phenotypic and genotypic characterization of acyclovir-resistant herpes simplex viruses from immunocompromised patients.免疫功能低下患者中阿昔洛韦耐药单纯疱疹病毒的表型和基因型特征
J Infect Dis. 1998 Aug;178(2):297-303. doi: 10.1086/515626.
9
Rapid screening tests for determining in vitro susceptibility of herpes simplex virus clinical isolates.用于确定单纯疱疹病毒临床分离株体外敏感性的快速筛查试验。
J Clin Microbiol. 1998 Aug;36(8):2389-91. doi: 10.1128/JCM.36.8.2389-2391.1998.
10
Survey of resistance of herpes simplex virus to acyclovir in northwest England.英格兰西北部单纯疱疹病毒对阿昔洛韦的耐药性调查。
Antimicrob Agents Chemother. 1998 Apr;42(4):868-72. doi: 10.1128/AAC.42.4.868.
Zotero 插件