Hansen G M, Paturzo F X, Smith A L
Section of Comparative Medicine, Yale University School of Medicine, New Haven, Connecticut, USA.
Lab Anim Sci. 1999 Aug;49(4):380-4.
Two serotypes of autonomously replicating parvoviruses infect laboratory mice. Genome regions coding for the nonstructural proteins of minute virus of mice [MVM] and mouse parvovirus [MPV] are almost identical, whereas capsid-coding sequences are divergent. We addressed these questions: Does humoral immunity confer protection from acute infection after challenge with homotypic or heterotypic parvovirus, and if it confers protection against acute MPV infection, does it also protect against persistent MPV infection?
Infant mice without maternal antibody or antibody to MVM or MPV and young adult mice given normal mouse serum or antibody to MVM or MPV were challenged with homotypic or heterotypic virus. In situ hybridization with target tissues was the indicator of infection.
Humoral immunity failed to confer protection against acute heterotypic parvovirus infection. In passive transfer studies, MPV DNA was observed occasionally in lymph nodes, intestine, or the spleen of MPV-challenged mice given homotypic antibody and kept for 6 or 28 days. Variable proportions of mice given MPV antibody and homotypic challenge had viral DNA in lymphoid tissues 56 days after virus inoculation.
A mouse or colony that has sustained infection with MVM or MPV is probably fully susceptible to infection with the heterotypic virus.
两种自主复制型细小病毒血清型可感染实验小鼠。小鼠微小病毒（MVM）和小鼠细小病毒（MPV）编码非结构蛋白的基因组区域几乎相同，而衣壳编码序列则存在差异。我们探讨了以下问题：体液免疫能否在同型或异型细小病毒攻击后提供针对急性感染的保护？如果它能提供针对急性MPV感染的保护，那么它是否也能预防持续性MPV感染？
对无母源抗体或无MVM或MPV抗体的幼鼠，以及给予正常小鼠血清或MVM或MPV抗体的成年幼鼠进行同型或异型病毒攻击。用靶组织原位杂交作为感染指标。
体液免疫未能提供针对急性异型细小病毒感染的保护。在被动转移研究中，在给予同型抗体并饲养6天或28天的受MPV攻击小鼠的淋巴结、肠道或脾脏中偶尔观察到MPV DNA。给予MPV抗体并接受同型攻击的小鼠中，不同比例的小鼠在病毒接种56天后其淋巴组织中存在病毒DNA。
持续感染MVM或MPV的小鼠或群体可能对异型病毒感染完全易感。
